烧伤对不同性别小鼠肝脏和脾脏铁相关蛋白的影响  

The effect of burns on iron-related proteins in liver and spleen of different sex mice

作　　者：郭晓强[1] 郭佩[2] 张彩云[2] 刘卫[1] 

机构地区：[1]白求恩军医学院生物化学教研室,河北石家庄050081 [2]河北师范大学生命科学院,河北石家庄050016

出　　处：《西安交通大学学报（医学版）》2009年第3期362-365,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：全军十一五青年基金资助项目(No.06Q097)~~

摘　　要：目的研究不同性别小鼠肝脏和脾脏铁相关蛋白烧伤前后的变化。方法90℃水蒸气制备烧伤小鼠模型,采用比色法测定烧伤小鼠肝脏和脾脏中的铁含量,逆转录-聚合酶链式反应(RT-PCR)检测肝脏hepcidin和膜铁转运蛋白1(Fpn1)表达,使用Western检测了肝脏和脾脏Fpn1蛋白表达。结果烧伤后雄性小鼠脾脏铁含量升高,肝脏hepcidin表达升高同时肝脏和脾脏Fpn1蛋白含量减少。烧伤后雌性小鼠铁内稳态破坏程度较轻。结论铁相关蛋白的表达变化可能是引起烧伤后铁内稳态破坏的重要原因,不同性别小鼠铁相关蛋白表达差异可能暗示了雌激素的保护作用。Object after burns. Methods ve To investigate the changes of iron-related proteins in different sex mice before and The burn model in mice were prepared with 90 ℃vapor. Both liver and spleen iron contents were measured by colorimetric method. Hepatic hepcidin and Fpn1 mRNA content were determined with RT-PCR. The Fpn1 protein was determined with Western blot. Results After burns, the content of spleen iron was increased and the expression of hepatic hepcidin was decreased in male mice, and at the same time the Fpn1 of liver and spleen was reduced. The content of iron homeostasis abnormality in female mice was less serious. Conclusion The change of iron-related proteins after burns may be one cause of iron homeostasis abnormality. The differences of iron-related protein expression in different sex after burns suggest that estrogen has a potentially protective function.

关 键 词：烧伤 铁 HEPCIDIN 膜铁转运蛋白 

分 类 号：R556.3[医药卫生—血液循环系统疾病]