黄芪、三七不同剂量配伍对肾小球系膜细胞增殖的影响  

Effect on proliferation of glomerular mesangial cell by the dose compatible proportion between Radix Astrsgali and Radix Notoginseng in Tongmai Oral Liquid

作　　者：刘明平[1] 黄兆胜[2] 吴庆光[1] 韦品清[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510405 [2]广州中医药大学护理学院,广东广州510405

出　　处：《西安交通大学学报（医学版）》2009年第3期377-379,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30271605)~~

摘　　要：目的通过考察通脉口服液方中黄芪、三七不同剂量配伍对肾小球系膜细胞增殖的影响,筛选其最佳配伍剂量。方法采用血清药理学方法,制备不同剂量配伍的通脉含药血清,体外检测其对肾小球系膜细胞(GMC)增殖的影响。结果通脉口服液,黄芪、三七不同剂量配伍含药血清,无论在正常培养条件下还是在脂多糖(LPS)刺激条件下,均对GMC增殖有一定的抑制作用;各剂量配比组之间比较,通脉3∶1含药血清抑制作用优于通脉6∶1、1.5∶1含药血清。结论原方黄芪、三七剂量按3∶1配比具有一定的合理性。Objective To study the effect on proliferation of glomerular mesangial （GMC） cell by the dose compatible proportion between Radix Astrsgali and Radix Notoginseng in Tongmai Oral Liquid （TMOL） so as to select the optimal dose proportion. Methods We used serological pharmacological methods to prepare Decoction of Tongmai of different dose proportions and assay its effect on the proliferation of GMC to confirm the best dose compatible proportion between Radix Astrsgali and Radix Notoginseng of Decoction of Tongmai. Results Both TMOL and the different proportions between Radix Astrsgali and Radix Notoginseng （6 ：1, 3 ：1, 1.5：1） of Decoction of Tongmai could inhibit the proliferation of GMC cultured either in fetal cow serum （FCS） or in FCS plus lipoplysaccharide. The inhibitory effect of Decoction of Tongmai （3 ： 1） group was better than both Decoction of Tongmai 6 ： 1 and Decoction of Tongmai 1.5 ： 1. Conclusion The dose compatible proportion between Radix Astrsgali and Radix Notoginseng in TMOL was reasonable.

关 键 词：黄芪 三七 肾小球系膜细胞 配伍研究 

分 类 号：R289.5[医药卫生—方剂学]