痰液脱落细胞FHIT基因检测在肺癌早期诊断中的评价  

Evaluation of FHIT gene detection for early diagnosis lung cancer in sputum specimen

作　　者：庄鹏晖[1] 蒋晓刚[2] 潘承恩[3] 尚东[4] 

机构地区：[1]西安交通大学医学院第一附属医院胸外科,陕西西安710061 [2]西安交通大学医学院遗传与分子生物学系,陕西西安710061 [3]西安交通大学医学院第一附属医院老年外科,陕西西安710061 [4]西安交通大学医学院第一附属医院呼吸内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2009年第3期383-386,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关基金资助项目(No.2005K09-G1)~~

摘　　要：目的通过检测痰液脱落细胞FHIT基因微卫星不稳定性(microsatellite instability,MSI)和杂合性缺失(loss of heterozygosity,LOH),评价FHIT基因检测在肺癌早期诊断中的敏感性,探讨肺癌筛查的有效方法。方法以液基细胞学为基础,采集并分离高危人群痰液脱落细胞,提取DNA,检测FHIT基因MSI和LOH。结果在肺癌患者中出现MSI或LOH异常的阳性率在41.6%～49.5%之间,以D3S1300最高,达到49.5%,3个位点中至少1个位点出现微卫星异常为72.3%(n=73),至少2个位点出现微卫星异常为45.5%(n=46)。统计学分析表明,FHIT基因出现MSI或LOH在肺癌和非肺癌患者中的差异有统计学意义(P<0.05)。结论以液基细胞学为基础,检测痰液脱落细胞FHIT基因的MSI和LOH可以作为肺癌早期诊断的新途径。不同微卫星位点出现异常表现的具体形式有所不同,多位点联合检测可以提高诊断的敏感性和特异性。Objective By detecting microsatellite instability （MSI） and loss of heterozygosity （LOH） in FHIT gene in sputum specimen, we intended to evaluate the sensitivity of FHIT gene detection to early diagnosis of lung cancer and explore effective method to screen lung cancer. Methods Based on liquid-based cytology, we collected and isolated sputum specimens in high risk group, abstracted DNA, and detected MSI and LOH in FHIT gene. Results The positive rate of MSI or LOH at single locus was between 41.6% and 49.5% ; the site D3S1300 was the highest and it was 49.5%. At least 1 site out of 3 sites that had abnormal microsatellite accounted for 72.3% in lung cancer patients, two sites that appeared to change accounted for 45. 5%, which was significantly different compared to non-lung cancer patients （P〈0.05）. Conclusion Based on liquid-based cytology, detection of MSI and LOH in FHIT gene in sputum specimen may be a means of screening lung cancer. Different loci may have different abnormal appearance; combination detection in multi-locus might raise sensitivity, which could provide new means for lung cancer screening.

关 键 词：液基细胞学 痰液标本 微卫星不稳定性 杂合性缺失 肺癌诊断 

分 类 号：R446[医药卫生—诊断学]