^(131)I标记内皮抑素的方法学研究  

^(131)I Labeling of Endostatin

作　　者：张金赫[1] 尹吉林[1] 全江涛[1] 周进[1] 刘京[1] 王欣璐[1] 

机构地区：[1]广州军区广州总医院核医学科,广州510010

出　　处：《华南国防医学杂志》2009年第3期34-36,共3页Military Medical Journal of South China

基　　金：广东省医学科研基金项目(A2006502)

摘　　要：目的探索用131I标记内皮抑素(Endostatin,ES)的方法,并研究标记产物的体外稳定性及其生物活性。方法用Iodogen法对ES进行131I标记,用正交设计筛选最佳标记条件;对标记产物用Sephadex G-25层析柱进行分离纯化,并计算标记率,用纸层析法测放化纯;产物静置以观察其体外稳定性;用人脐静脉内皮细胞系ECV304观察其生物活性。结果Iodogen法最佳标记条件为ES20μg,Iodogen50μg,131I18.5MBq,反应时间1min,标记率可达65.0%。产物在体外稳定;细胞培养观察其对内皮细胞抑制率高于单用ES或131I,有显著性差异。结论用Iodogen法对ES进行131I标记简单高效,标记产物生物活性未受明显破坏。Objective To explore the method of ^131I labeling of endostatin （ES） and investigate the stability and biological activity of ^131I-ES in vitro. Methods Iodogen method was applied to label ES with ^131I. The best synthesis condition was determined by cross design. Labeling efficiency was measured by paper chromatography and column chromatography. The stability of ^131I-ES was observed at different times. Biological activity of ^131I-ES was observed in human umbilical vein endothelial cell line ECV304. Results The best experimental condition was： 20 μg of ES, 50μg of Iodogen, 18. 5 MBq of ^131I, 1min of reaction time. The labeling efficiency can be achieved to 65.0%. ^131I-ES was stable in vitro and its inhibitory rate on endothelial cell was better than ES or ^131I singly. Conclusion ^131I labeling of ES is simple and effi- cient. The biological activity of ^131 I-ES is better than ES.

关 键 词：内皮抑素 131I 放射性同位素标记 

分 类 号：R817.4[医药卫生—影像医学与核医学]