表面活化PLA纤维增强胶原多孔支架材料  

Porous Collagen Scaffold Reinforced by Surface Activated PLA Fiber

作　　者：刘曦[1] 黄长斌[1] 冯玉杰[1] 樊渝江[1] 

机构地区：[1]四川大学生物材料工程研究中心,四川成都610064

出　　处：《临床医学工程》2009年第6期1-3,共3页Clinical Medicine & Engineering

基　　金："863"计划资助项目(项目编号:2006AA02A125)

摘　　要：目的用表面活化PLA纤维增强胶原海绵,制备具有良好力学性能的组织工程支架。方法用己二胺处理PLA纤维,在表面引入氨基,经过戊二醛处理制备醛基活化的PLA纤维。将表面活化PLA纤维与I型胶原溶液均匀混合,经冷冻干燥和交联得到复合多孔胶原海绵。考察其形貌,物理化学性质和细胞毒性,并评价细胞在支架上的黏附和生长情况。结果活化PLA纤维可均匀分散在胶原中,明显增强了胶原海绵的抗压缩能力,而不影响其多孔结构。制得的多孔支架材料不显示细胞毒性,L929细胞在活化PLA纤维增强支架上的粘附优于纯胶原海绵和纯PLA纤维增强胶原海绵,组织学染色显示其内部细胞增殖分布更好。结论表面活化PLA纤维增强胶原海绵是一种有前景的组织工程支架材料。Objective Porous collagen sponges were reinforced using surface activated PLA fibers to prepare scaffold materials with improved mechanical properties for tissue engineering. Method The activated PLA fiber were obtained by successive treatment of PLA fiber with hexanediamine and glutaraldehyde to introduce the aldehyde groups on the surface of PLA fiber. The treated fibers were mixed with collagen solution, frozen-dried, and crosslinked to obtain the reinforced porous collagen sponge. Result The evaluation results showed that the scaffold materials had no cytotoxicity. The improved mechanical properties of the scaffold benefited the infiltration, attachment and distribution ofL929 mouse fibroblast cells in the pores inside the scaffold. Conclusion Collagen sponge reinforced with surface activated PLA fibers is expected as a promising scaffold material for tissue engineering.

关 键 词：胶原海绵 增强 PLA纤维 表面活化 

分 类 号：R318.08[医药卫生—生物医学工程]