常通口服液对大鼠正常及粘连腹膜成纤维细胞周期的影响  

Effect of ChangTong oral liquid on the cell cycle of cultured fibroblasts from normal peritoneum and adhesion

作　　者：王春霞[1] 曾煦欣[2] 霍启录[1] 侯连兵[1] 

机构地区：[1]南方医科大学南方医院,广州510515 [2]佛山科学技术学院,佛山510000

出　　处：《中华中医药杂志》2009年第7期879-881,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30873271);粤港关键领域重点突破项目(No.2007A032702002)

摘　　要：目的:探讨常通口服液(CTOL)对体外培养的正常腹膜成纤维细胞(NF)及粘连腹膜成纤维细胞(AF)周期的影响。方法:雄性SD大鼠15只,随机分为正常血清组、CTOL中、高剂量组。大鼠灌服相应剂量的药物7d后采血,分离药物血清;采用组织块贴壁培养法进行成纤维细胞培养,本实验所用细胞为第3-8代;取对数生长期的NF及AF,分别加入正常对照血清,CTOL中、高剂量含药血清,培养12、24、48h后,流式细胞仪检测分析成纤维细胞周期。结果:与正常血清组比较,CTOL中剂量作用12、24、48h对NF的DNA合成前期(G1期)细胞比例无显著影响,而高剂量含药血清组的NF的G1期细胞比例显著增加(P<0.001);CTOL中、高剂量含药血清组的AF在12、24、48h的G1期细胞比例均显著升高(P<0.01)。而S期及G2期细胞比例呈下降趋势,但各组间无显著性差异。结论:CTOL可以抑制体外培养的NF和AF增殖,调整细胞周期。CTOL对成纤维细胞增殖的抑制率之间存在剂量依赖关系,而且,药物血清对AF作用更敏感。Objective： To explore the effect of ChangTong oral liquid （CTOL） on cell cycle of cultured fibroblasts from normal peritoneum（NF） and adhesion（AF）. Methods： 15 SD male rats were randomly divided into 3 groups： normal serum group, 2 CTOL groups （medium and high doses）. After administration of medication at the different dosages for 7 days, serums were obtained from the abdominal arteries; the prepared tissues were cultured until outgrowth of fibroblasts. In our study, the passage 3-8 ceils were used in order to maintain comparability; The fibroblasts in desired condition were plated in 10% FBS, then divided into three groups： rat serum normal group, CTOL groups （ medium and high dose ） . Cells were cultured respectively for 12, 24 and 48 h, and analyzed by flow cytometer. Results： Compared with serum normal group of NF, CTOL medium dosage could increase the proportion of NF in the G1 stage at 12, 24 and 48h, but only CTOL high dosage had significance （P〈0.001）. CTOL Medium and high dose could not markedly reduce the proportions of cell at S and G2 stages. Compared with serum normal group of AF, CTOL medium and high dosage could significantly increase the proportion of AF at the G1 stage at 12, 24 and 48h （P〈0.01）, but could not markedly reduce the proportions of cells at S and G2 stages. Conclusions： CTOL could restrain the proliferation and regulate cell cycle of fibroblasts. AF was more sensitive to medication than normal ones, coming out to be dose-dependent.

关 键 词：常通口服液 成纤维细胞 细胞增殖 流式细胞 

分 类 号：R285.5[医药卫生—中药学]