黄芩药材中主要黄酮类成分含量测定及指纹图谱研究  

HPLC quantitative analysis and fingerprint study of the chief flavonoids in scutellaria baicalensis

作　　者：周永妍[1] 程秀民[1] 于海英[1] 王莹[1] 

机构地区：[1]山东大学药学院,济南250012

出　　处：《中华中医药杂志》2009年第7期882-885,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家支撑计划资助(No.2006BAI06A01-05)

摘　　要：目的:建立不同产地黄芩药材指纹图谱,并测定主要黄酮类成分的含量来评价药材质量。方法:采用岛津SHIMADZU VP-ODS柱(250mm×4.6mm,5μm),以0.1%枸橼酸-乙腈为流动相进行梯度洗脱,流速1.0ml/min,检测波长为276nm及200nm-400nm,应用二极管阵列检测器检测。所得不同样品的指纹图谱用相似度评价软件进行分析。结果:确定了17个共有峰,建立了黄芩药材的指纹图谱。但各主要成分的含量有较大的差异,其中沂源县的扦插黄芩中的黄芩苷含量最高,高达18.32%;东北野生黄芩中的黄芩素和汉黄芩素含量最高,分别为2.31%和0.73%。结论:此方法简单、准确、重复性好,峰的分离度较好,可作为黄芩质量控制的方法。Objective： To set up the HPLC fingerprints of Scutellaria baicalensis collected from different regions and develop a HPLC method for the quantitative analysis of the chief Flavonoids in Scutellaria baicalensis.Methods：The HPLC separation was performed on SHIMADZU VP-ODS column （250mm×4.6mm, 5μm ） , gradient ehited using 0.1% citric acidacetonitrile solvent system and detected at 276nm and 200nm to 400nm with a DAD detector. The flow rate was 1.0ml/min. The fingerprints of different samples were compared with similarity evaluation software. Results The HPLC fingerprints of Scutellaria baicalensis was set up, in the fingerprints, 17common peaks of flavonoids were confirmed. But the contents of the chief Flavonoids had a big difference, in which the baicalin had the biggest content（18.32%） in cottage Scutellaria baicalensis of Yiyuan County; the baicalein and wogonin had the biggest content（2.31%＆0.73%） in wild Scutellaria baicalensis of Northeastern China. Conclusion： This method showed high precision, good repeatability, stability and all of the content were separated well, so it can be useful as the means to assess the quality of Scutellaria balicalensis.

关 键 词：黄芩苷 黄芩素 汉黄芩素 含量测定 指纹图谱 

分 类 号：R284[医药卫生—中药学]