反相高效液相色谱法测定墨旱莲中齐墩果酸的含量  

Determination of oleanolic acid in Herba Ecliptae by reversed-phasehigh performance liquid chromatography

作　　者：邵振中[1,2] 贾晓斌[1,2] 施峰[1,2] 陈彦[1] 金晓勇[1] 宋师花[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统应用技术重点研究室,南京210028 [2]江苏大学药学院,镇江212013

出　　处：《中华中医药杂志》2009年第7期924-926,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江苏省中医药领军人才(贾晓斌)项目(No.2006);江苏省自然科学基金(No.BK2006155)

摘　　要：目的:建立测定墨旱莲中齐墩果酸的含量的高效液相色谱法。方法:采用Phenomene×C18柱(4.6mm×250mm,5μm),流动相为乙腈-0.1%磷酸水溶液(90:10),检测波长210nm,流速1.0ml/min,柱温为30℃,进样量为20μl。结果:齐墩果酸在0.569-3.414μg内峰面积与其质量浓度有良好的线性关系(r=0.9999);平均加样回收率为98.40%(RSD=2.44%)。结论:该方法简便、快速、准确,可作为墨旱莲质量控制的一个有效方法。Objective： To develop a method of RP-HPLC for the quantitative analysis of oleanolic acid in Herba Ecliptae. Methods： Oleanolic acid was separated on Phenomenex C18 （4.6 mm×250 mm, 5 μm） using a mixture of acetonitrile and 0.1% phosphoric acid solution（90：10） as the mobile phase and detected at 210 nm; The flow rate was 1.0 ml/min and 20 μl. sample solution was injected every time. Results： There was good liner relationship between the peak area and concentration at ranges of 0.569-3.414μg（r=0.999 9） for oleanolic acid; the average recovery rate of oleanolic acid was 98.40% （ RSD=-2.44% ） . Conclusion： The established method was rapid and simple with good accuracy and reproducibility for the determination of oleanolic acid ,the method was suitable for the quality control of Herba Ecliptae.

关 键 词：反相高效液相色谱法 齐墩果酸 墨旱莲 

分 类 号：R284[医药卫生—中药学]