桂枝茯苓丸对荷瘤小鼠瘤组织Survivin及P21^(waf/cip)的影响  

Effect of Guizhi Fuling Bolus on expression of Survivin、P21^(waf/cip) in tumor tissue of tumor-bearing mice

作　　者：孙艳[1] 王琪[1] 

机构地区：[1]齐齐哈尔医学院病原生物教研室,齐齐哈尔161006

出　　处：《中华中医药杂志》2009年第7期936-938,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江省教育厅资助项目(No.11511458)

摘　　要：目的:探讨桂枝茯苓丸抑瘤作用的机制。方法:取30只小鼠,每只鼠右侧腋下皮下注射0.2ml的S180瘤细胞悬液,将其随机分为模型组、中药桂枝茯苓丸(GuizhiFuling Bolus,GFW)组、环磷酰胺(cyclophosphane,CY)组,每组10只,另取10只未荷瘤小鼠作为正常对照。模型组用生理盐水灌胃,每天0.2ml/10g;中药组用GFW给小鼠灌胃,每天0.2ml/10g;环磷酰胺组于小鼠腹腔注射CY,每天20mg/kg。各组小鼠均于接种后次日给药,每天给药1次,连续10d后,小鼠脱颈处死,取腋下实体瘤,计算抑瘤率。免疫组化法测定P21waf/cip蛋白表达,原位杂交法检测存活素(Survivin)mRNA表达。结果:GFW抑瘤率为38.93%,GFW上调肿瘤细胞P21waf/cip表达,下调Survivin表达。结论:GFW可抑制肿瘤细胞生长,其机制可能与上调P21waf/cip及下调Survivin mRNA表达密切相关。Objective： To Study the molecular mechanism of Guizhi Fuling Bolus（GFW） controlling the tumor growth. Methods： Each of 30 mice received 0.2ml S180 tumor cell suspension subcutaneously in the right axilla.The mice were randomly divided into model group,GFW group and Cyclophosphane（CY） group and with 10 mice in each group.Another ten mice without cancer were used as control.The model group received intragastric normal saline,0.2ml·10g^-1·d^-1;the GFW group was given intragastric administration, 0.2ml·10g^-1·d^-1 whilst the CY group received intraperitoneal injection of CY at 20mg·kg^-1·d^-1.The medicines were given to the mice in each group after they were inoculated, once a day for 10 days.The mice were sacrificed and the tumor removed,the S180 mice sarcoma model was used to detect the inhibiting effects of GFW. The protein expressions of P21^waf/cip and mRNA expression of Survivin were evaluated by and in situ hybridization. Results： For S180 sarcoma, the inhibiting ratio of GFW was 38.93%. Survivin mRNA was markedly declined in the GFW group when compared with the model group （P 〈 0.01） ,while the positive expression of P21^waf/cip was obviously higher in the GWF group than in the model group （P 〈 0.01）. Conclusion： GFW controls the tumor cell growth, its mechanism maybe closely related with adjusting expression levels of P21^waf/cip and Survivin gene,

关 键 词：桂枝茯苓丸 SURVIVIN P21waf/cip 

分 类 号：R285.5[医药卫生—中药学]