重组人粒细胞集落刺激因子经鼻给药对脑梗死大鼠的脑保护作用  

Neuroprotective effects of rhG-CSF on brain given intranasal in cerebral infarct rats

作　　者：何美清 孙保亮[2] 张颜波[2] 

机构地区：[1]山东省泰山慢性病医院,泰安271000 [2]泰山医学院附属医院神经内科

出　　处：《中华行为医学与脑科学杂志》2009年第6期532-534,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金资助项目（30770759）;泰安市科技发展计划项目资助（20064019）

摘　　要：目的探讨重组人粒细胞集落刺激因子（rhG-CSF）鼻腔给药对脑梗死大鼠的脑保护作用。方法线栓法制备大鼠大脑中动脉阻塞模型，缺血2h再灌注，皮下及鼻腔给予rhG—CSF（60μg/kg）。将动物分为正常组、假手术对照组、脑梗死组、脑梗死＋皮下注射rhG—CSF组、脑梗死±经鼻生理盐水组、脑梗死＋经鼻rhG-CSF组，每组6只。于术后3d进行神经功能评分及FasL免疫荧光检测。结果除正常组、假手术对照组外，其余各组动物均于术后出现不同程度的神经功能缺损，尤以脑梗死组、脑梗死＋经鼻生理盐水组最为严重，神经功能评分最高[（10．20±1．85）分，（10．30±1．76）分]，海马FasL阳性细胞数最多[（41．17±3．25）个，（41．00±2．76）个]，差异无显著性（P〉0．（15），rhG-CSF皮下给药组大鼠神经功能评分降低[（5．67±1．32）分，P〈0．01]，海马FasL表达减少[（32．67±1．97）分，P〈0．01]，rhG-CSF经鼻给药组大鼠神经功能评分进一步降低[（4．（30±0．93）分，P〈0.05]，海马FasL表达进一步减少[（19．50±1．05）个，P〈0．01]。结论rhG-CSF经鼻给药能预防和修复脑梗死大鼠的神经功能缺损，通过抑制FasL表达发挥脑保护作用。Objective To explore the protective effect of rhG-CSF given intranasally on cerebral infarct rats by observing the neurological dysfunction and the expression of Fas ligand （FasL） in hippocampus of cerebral infarct rats. Methods Middle cerebral artery occlusion （ MCAO ） model rats were established by nylon strand, reperfuse 2 hours later, and give rhG-CSF through subcutaneous and intranasal way. The rats were divided into the nermal group,the sham-operated control group（sham） , MCAO group, MCAO ± NS given intranasally group（NS） , MCAO ± rhG-CSF given subcutaneously group, and MCAO ± rhG-CSF given intranasally group each group had 6 rats. At the time of 3d after reperfusion,neurological severity scores （NSS） test was performed and the expression of FasL was detected via immunohistochemical staining in collateral hippocampus. Results Neurological dysfunc- tion appeared in all groups except for the normal and the sham group. The dysfunction of the MCAO and the NS group was the most serious,the NSS was the highest （ 10.20 ± 1.85,10.30 ± 1.76） , the number of FasL positive cells was the most（41.17 ± 3.25,41. 00 ± 2.76） , and there was no obvious difference between the two groups （P 〉 0.05 ） ; the NSS and FasL positive cells decreased in the subcutaneous group （ 5.67 ± 1.32, P 〈 0. 01 ; 32.67 ± 1.97, P 〈 0.01 ） and decreased further more in the intranasal group（4.00 ± 0.93, P 〈 0.05 ; 19.50 ± 1.05, P 〈 0. 01 ）. Conclusions rhG-CSF given intranasally can relieve the neurological dysfunction of cerebral infarct rats, and brain cells are thereby protected by resisting the expression of FasL.

关 键 词：粒细胞集落刺激因子 脑梗死 鼻腔给药 

分 类 号：R96[医药卫生—药理学]