电针对海洛因成瘾大鼠戒断后焦虑情绪及中央灰质β-内啡肽表达的影响  

Effect of acupuncture on anxiety mood and the expression of β-EP in peraqueductal grey of herion withdrawal rats

作　　者：王桂华[1] 潘贵书[2] 张新勇[3] 

机构地区：[1]烟台滨州医学院生理教研室,264003 [2]遵义医学院生理教研室 [3]淮安市第二人民医院神经内科

出　　处：《中华行为医学与脑科学杂志》2009年第6期535-537,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：贵州省委组织部人才基金项目（[黔人领函2007]09号）

摘　　要：目的观察电针对海洛因成瘾戒断大鼠情绪及中央灰质（periaqueductalgrey，PAG）β-内啡肽（β-endorphine，β-EP）表达的影响。方法通过剂量递增方法，建立海洛因依赖模型。简单随机抽样将大鼠分为对照组、成瘾组、戒断组、针刺组。高架十字迷宫实验判断大鼠的情绪状态；免疫组化的方法检测PAGβ-EP的表达；观察戒断后，针刺足三里和三阴交穴对戒断大鼠情绪和PAGβ-EP表达影响。结果海洛因成瘾戒断后大鼠各项焦虑指标：开臂停留时间占总时间百分比（OT％）、开臂进入次数占进臂总次数百分比（OE％）、探头次数[分别为（12．5±4．3）％，（17．1±6．7）％，（5．7±2．0）次]低于对照组[分别为（26．8±8．7）％，（32．4±6．0）％，（12．2±4．0）次]，差异具有显著性（P值分别为0．003，0．018，0．003），β-EP表达产物的平均光密度值（206．1±23．1）高于对照组（186．2±15．3），差异具有显著性（P=0．041）；戒断组大鼠给予针刺后OT％、OE％、探头次数各项焦虑指标值[分别为（26．5±8．7）％，（31．8±7．7）％，（9．9±3．1）次]增高与对照组比较差异无显著性（P值分别为0．920，0．816，0．122），β-EP表达产物的平均光密度值（185．3±11．4）与对照组（186．2±15．3）比较亦差异无显著性（P=0．891）。结论针刺能减轻戒断大鼠的焦虑情绪且具有促进PAG内源性β-EP表达的作用，这可能是针刺改善戒断大鼠的焦虑情绪的一个重要机制之一。Objective To explore the effect of Electroacupuncture on anxiety mood and periaqueductal grey （PAG） β-endorphine expression in rats with morphine abstinence syndrome. Methods The model of heroinaddicted rat was firmly established by the subcutaneous injection of gradually-increased dosages of heroin. Rats were randomly divided into control group, herion-addict group, withdrawal group, eleetroaeupuneture group. Anxiety mood of experimental rats were assessed by Elevated Plus Maze test. Expression of β-EP in PAG was detected by immunohistochemstry. The effect of eleetroacupuncture at bilateral zusanli and sanyinjiao on anxiety mood and expression of β-EP in PAG was observed in rats with herion abstinence symptom. Results The anxiety index in rats with herion abstinence symptom：the value of OT% , OE% and head-dips times was （12.5 ± 4.3 ） % , （17.1 ± 6.7 ） % and （ 5.7± 2.0） times respectively, lower than that in control group [ （ 26.8 ± 8.7 ） % , （ 32.4 ± 6.0 ） % and （12.2± 4.0 ） times respectively ], there existed significant difference （The value of P was 0. 003,0. 018 and 0. 003 respectively）. Meanwhile, The expression of β-EP-positive neurons （ 206. 1 ± 23. 1 ） was significantly higher than the control（ 186.2 ± 15.3 ）. There existed significant difference （P = 0. 041 ）. Electroacupuncture could increase OT% , OE% , head-dips times [ （ 26.5 ± 8.7 ） %, （ 31.8 ± 7.7 ） % and （ 9.9 ± 3. l ） times respectively ] in rats with herion abstinence symptom, which eomparied with control group were not statistically distinctive （The value of P was 0. 920,0. 816 and 0. 122 respectively）. The expression of β-EP（ 185.3± 11.4）in PAG increased, which eomparied with control group（ 186.2 ± 15.3 ）were not statistically distinctive（P= 0. 891 ）. Conclusion Ele ctroacupuncture could inhibit the abstinence syndrome effectively. And there is obvious promotion of β-EP expression in PAG. This is probably one of underlying mechanisms for electroacupu

关 键 词：电针 海洛因 成瘾 焦虑 中脑导水管周围灰质 Β-内啡肽 

分 类 号：R245[医药卫生—针灸推拿学]