产头孢菌素酶大肠埃希菌的随机扩增多态性DNA分型研究  

Genotyping of cephalosporinase(AmpC)β-lactamases-Producing Escherichia coli by RAPD

作　　者：徐令清[1] 漆涌[1] 宋明胜[1] 陈辉[1] 李翔[1] 徐文[1] 伍勇[1] 

机构地区：[1]中南大学湘雅三医院检验科,湖南长沙410013

出　　处：《国外医学（医学地理分册）》2009年第2期89-92,共4页Foreign Medical Sciences：Section of Medgeography

基　　金：湖南省卫生厅资助项目(B2005-074)

摘　　要：目的对产头孢菌素酶大肠埃希菌的耐药现状进行分析研究,对耐药株进行基因分型流行病学研究。方法对临床分离的157株大肠埃希菌进行头孢菌素酶(AmpC)检测,采用K—B琼脂扩散法,按照美国CLSI规定判断结果,判断细菌的耐药性;对产AmpC酶菌株以随机扩增多态性DNA分型法(RAPD)进行基因分型;接合试验证实酶基因有无可转移性。结果产AmpC酶大肠埃希菌占8.9%(14/157);14株产AmpC酶菌株经过RAPD扩增分为13型。结论本院已经发现产质粒介导AmpC酶菌株,其耐药性能够水平传播。药敏检测结果显示对大多数新型广谱β-内酰胺类抗生素耐药,对亚胺培南敏感。RAPD证实他们大多来自不同的克隆株。RAPD是从分子水平对耐药细菌进行流行病学研究的一种经济、快速、可靠的基因分型方法。Objective To investigate the current resistance and to estimate the prevalence trend of AmpC β-lactamase producing Escherichia coli. Methods Drug resistance of 157 strains were detected by Kirby-Bauer disc agar diffusion test. AmpC-producing Escherichia coli strains were genotyped by Random Amplified polymorphic DNA （RAPD）.Results The ratio of AmpC-producing strains was 8.9% （4/157）. Fourteen strains of AmpC- producing Escherichia coli were divided into 13 types by RAPD. Conclusions Plasmid--mediated cephalosporinase isolates had been found in our hospital, and they could transfer resistance to other isotlates. Sensitivity test shows that they are resistant to the most new broad β-1actamas antibiotics but sensitive to imipenem. RAPD is an economic, quick and credible method for epidemic analysis.

关 键 词：大肠埃希菌 头孢菌素酶 随机引物聚合酶链反应 

分 类 号：R466[医药卫生—临床医学]