二色胡枝子组培再生体系优化的研究  

Studies on optimizing Lespedeza bicolor regeneration system

作　　者：黄天伟[1] 李伟[1] 李明[1] 陈晓阳[1,2] 

机构地区：[1]北京林业大学林木花卉遗传育种教育部重点实验室,北京100083 [2]华南农业大学林学院,广东广州510642

出　　处：《河北林果研究》2009年第2期117-120,共4页Hebei Journal of Forestry and Orchard Research

基　　金：国家高技术研究发展计划(863计划)子项目"豆科饲料型灌木抗逆新品种选育"(2002AA241111)部分内容

摘　　要：为了实现二色胡枝子高效再生体系的优化,以二色胡枝子为材料,研究了6-BA、2,4-D、NAA等因素对其子叶节再生、节段扩繁和试管苗生根的影响。试验结果表明:子叶节再生最佳培养基为MS+6-BA0.1 mg/L;最佳增殖培养基为MS+6-BA0.1 mg/L+NAA0.01 mg/L;IBA,NAA对二色胡枝子试管苗的生根作用有影响,最佳生根培养基为1/2MS+IBA0.5 mg/L或者1/2MS+NAA0.1 mg/L。In order to optimize the effective regeneration system of Lespedeza bicolor, the effects of 6- BA,2,4- D, NAA on cotyledonary node regeneration,proliferation and rooting of Lespedeza bicolor were studied. The results showed that the optimum regeneration medium was MS ＋ 6 - BA 0. 1mg,/L. MS ＋ 6 - BA 0.1 mg/L ＋ NAA 0.01 mg/L was an optimum proliferation medium. IBA and NAA played an important role in rooting and the optimum roofing medium was 1/2MS ＋ IBA 0.5 mg/L or 1/2MS ＋ NAA0.1mg/L.

关 键 词：二色胡枝子 再生体系优化 增殖 生根 

分 类 号：S722.3[农业科学—林木遗传育种]