半乳糖苷化修饰葡萄糖氧化酶提高其热稳定性  

Modification of glucose oxidase with galactosylation for enhancing its thermostability

作　　者：殷海波[1] 贾楠[1] 李琳子[1] 郑小郎[1] 李任强[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2009年第3期305-309,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：暨南大学自然科学基金(640073)

摘　　要：利用一种单胺化的半乳糖衍生物,在炭化二亚胺的催化下,对葡萄糖氧化酶(GOD)进行糖苷化修饰,并利用蓖麻凝集素亲和层析分离已修饰的和未修饰的酶.修饰后的酶经荧光光谱和吸收光谱分析,证实其结构发生了变化.酶活性检测证明了修饰后GOD的热稳定性增加,在较高温度如在55℃下酶活性提高了7.28倍.本研究为拓宽GOD的应用奠定了基础.A monoaminated galactose derivative was attached to glucose oxidase （GOD） to modify it via a carbodiimide-catalyzed reaction. Ricinus agglutinin affinity chromatography was first adopted to soparate the modified and non-modified enzyme. The modified GOD was confirmed by absorption and fluorescence spectroscopy. The thermostability of modified GOD was enhanced, based on the examination of enzymatic activity. Under a higher temperature, such as at 55℃, the catalytic activity of modified GOD has been enhanced as high as 7.28 folds.

关 键 词：葡萄糖氧化酶 半乳糖 化学修饰 热稳定性 

分 类 号：Q814[生物学—生物工程]