骨髓间充质干细胞静脉移植对扩张型心肌病大鼠毛细血管生成的影响  

Influence of Mesenchymal Stem Cells Vein Injection Transplantation on Angiogenesis in Adriamycin-induced Cardiomyopathy Rats

作　　者：陈蓉[1] 张国辉[1] 蒋世忠[1] 汪健飞[1] 李卫东[1] 真亚[1] 郭俊芳[1] 张建[1] 鲍中华[1] 

机构地区：[1]江苏省镇江市第一人民医院心内科,212002

出　　处：《中国微循环》2009年第3期161-165,223,共6页Journal of Chinese Microcirculation

摘　　要：目的观察骨髓间充质干细胞(MSCs)经静脉途径移植对阿霉素诱导的扩张型心肌病(DCM)大鼠心功能的影响。方法建立大鼠阿霉素性心肌病模型,随机分为三组:DCM空白组(B组)、静脉移植组(C组)和静脉对照组(D组),另设正常对照组(A组)。经尾静脉注射在体外扩增并DAPI标记的骨髓间充质干细胞和培养基至C组和D组大鼠,A组和B组大鼠不干预。移植后8周心脏彩超观察左室舒张末径(LVDD)、左室射血分数(LVEF),多导生理记录仪观察左室最大压力上升速率(+LVdp/dtmax)、左室最大压力下降速率(-LVdp/dtmax),并分析组织病理学特征。结果细胞移植后8周,C组LVEF高于B组和D组;LVDD稍低于B组和D组;+LVdp/dtmax明显高于B组,也显著高于D组;-LVdp/dtmax明显高于B组和D组;C组心肌中可见带有蓝色荧光的DAPI标记的骨髓MSCs,心肌胶原容积百分比低于B组和D组,毛细血管计数高于B组和D组;C组VEGF水平明显高于B组和D组。结论骨髓MSCs静脉移植治疗阿霉素诱导的扩张型心肌病大鼠,提高VEGF水平,促进心肌毛细血管新生,减少胶原纤维,改善心功能。Objective To observe the influences of mesenchymal stem cells（MSCs） on heart functions of rats which have dilated adriamycin - induced cardiomypathy（DCM） by vein injection. Methods Rat models of adriamycin - induced cardiomyopathy were established. The rats were divided randomly into three groups： DCM group（ group B）, vein transplantation group（ group C） and vein control group（ group D）, with an additional normal control group（ group A）. Group C and group D were injected MSCs and equivalent volume culture medium through tail vein respectively, while group A and group B were not treated. 8 weeks after transplantation, the left ventricular dimension at end- diastole（LVDD） and ejection fraction（LVEF） were measured with eehocardiography; maximum left ventricular developed pressure increase rate（ ＋ LVdp/dtmax） and decrease rate（ - LVdp/dtmin） were recorded with continuous physiological monitors; and histopathologieal study was done in the 9th week after transplantation. Results 8 weeks after transplantation, LVEF increased in group C compared with pre - transplantation（0.86 ± 0.01 vs. 0.82 ±0.03, P 〈 0.05）, and it was higher than that in group B and group D; LVDD slightly decreased in group C compared with pre -transplantation, and it was lower than that in group B and group D; ＋ LVdp/dtmax was higher than that in group B and group D, - LVdp/dtmin was also higher than that in group B and group D. The DAPI labeled cells were found at the tissue section with their nuclears blue in the 9th week. Compared with group B and group D, collagen volume fraction（CVF） in group C was lower, and capillary amount was remarkably higher. The VEGF level of group C is apparently higher than that of group B and group D. Conclusion MSCs transplantation can increase VEGF level, elevate ± LVdp/dtmax, promote angiogenesis in adriamycin -induced cardiomyopathy rat models and reduce collagen volume fraction, thus improve the functions of heart.

关 键 词：骨髓间充质干细胞 细胞移植 扩张型心肌病 心功能 

分 类 号：R392.4[医药卫生—免疫学]