大鼠急性内囊出血后血液流变特性经时变化的研究  

Studies on Dynamic Changes of Hemorrheological Properties on Rats with Acute Internal Capsular Hemorrhage

作　　者：张丽敏[1] 金学隆[1] 王世忠[2] 李之烨[1] Mizoguchi Kazushige 

机构地区：[1]天津医科大学基础医学院生理教研室,天津300070 [2]天津医科大学基础医学院组胚教研室,天津300070 [3]Department of Geriatric Medicine,National Institute for Longerity Sciences,National Center for Geriatrics and Gerontology

出　　处：《中国微循环》2009年第3期172-175,共4页Journal of Chinese Microcirculation

基　　金：天津市自然科学基金重大项目(08JCZDJC25000)

摘　　要：目的观察大鼠急性内囊出血后血液流变特性的经时变化,总结变化规律,分析其对微循环的影响。方法将大鼠随机分为假手术组及出血组,后者又分为出血后3、6、12、24h四个亚组,每组6只。尾尖剪断取自体血0.1ml定位注射至大鼠右侧内囊,假手术组除不注血外,其余操作同出血组。假手术组即刻取血,出血组分别于各时间点取血做血液流变学检测。结果各出血组与假手术组比较,血液流变学各指标总体水平呈上升趋势(P<0.05);出血各组间比较,全血低切黏度在出血后3～12h明显升高,全血高切黏度、血浆黏度在24h升高最明显,红细胞压积(HCT)、红细胞聚集指数(EAI)及纤维蛋白原(Fib)含量随时间推移逐渐升高,前者在24h达高峰,后两者高峰时间为出血后12～24h。结论大鼠急性内囊出血后不同时段的血液流变学参数发生了有统计学意义的改变,将影响中枢神经系统的微循环功能。Objective To observe the changes of hemorrheologlcal properties in rats with acute internal capsula/hemorrhage, summarize the regularity and analyze its influence on organ microcirculation. Methods 30 normal adult Wistar rats were randomly divided into sham group and cerebral hemorrhage（CH） group, the latter was subdivided into 4 groups as 3, 6, 12, 24 h groups. Cut the tail to get 0.1 ml autoblood and inject it into the right intemal capsule, the operation on the sham group was the same as the CH sub groups except for no blood injection. Draw blood from the sham group immediately and from the CH group at different time point for hemorrheology detection. Results Compared with the sham group, the level of hemorheological parameters in CH sub groups increased generally（ P 〈 0.05）. When CH subgroups were compared, low shear blood viscosity raised obviously from 3 h to 12 h. High shear blood viscosity and plasma viscosity raised the most obviously at 24 h. The hematocrit（HCT）, erythrocyte aggregation index（EAI） and fibrinogen（Fib） contents increased gradually as time went on. HCT reached the peak at 24 h. The peak time of EAI and Fib is from 12 h to 24 h. Conclusion There were some statistical changes of hemorheological parameters at different time points of CH rats, which influenced the microcirculation function of central nervous system.

关 键 词：脑出血 血液流变学 微循环 

分 类 号：R331[医药卫生—人体生理学]