转crylAb基因克螟稻对根际可培养细菌类群的影响  

The effects of crylAb transgenic rice on the culturable bacterial flora in the rhizospheres

作　　者：杨保军[1] 唐健[1] 江云珠[1] 彭于发[2] 

机构地区：[1]中国水稻研究所,杭州310006 [2]中国农业科学院植物保护研究所,北京100094

出　　处：《生态学报》2009年第6期3036-3043,共8页Acta Ecologica Sinica

基　　金：国家重点基础研究发展计划(973)资助项目(2001CB109004)

摘　　要：田间栽培条件下,研究了转crylAb基因克螟稻1(KMD1)、克螟稻2(KMD2)及其亲本常规晚粳秀水11(XS11)不同生育期、不同根际部位(包括根际土壤、根表面和根内)可培养细菌类群数量、组成和动态变化。结果表明,各品种不同生育期根际平均细菌数量以拔节期最多,抽穗期最少;转Bt稻KMD1和KMD2根际平均细菌数量均显著高于XS11,且根表面和根内平均细菌数量也显著高于XS11,而根际土壤中平均细菌数量则低于XS11;试验共纯化菌株303个,经鉴定归于20个属,各品种根际出现的细菌类群大多为相同属,但数量有异,其中KMD1、KMD2根际的葡萄球菌属(Staphylococcus)、棒杆菌属(Corynebacterium)和壤霉菌属(Agromyces)均显著高于XS11,而XS11根际的束毛球菌属(Trichococcus)显著高于KMD1、KMD2;群落多样性分析表明,各水稻品种根际细菌群落的优势度指数C、多样性指数H′和均匀度指数J差异均不显著,表明转crylAb基因水稻对土壤细菌群落有一定影响,与水稻生育期和根际部位有关。In order to investigate the effects of transgenic Bacillus thuringiensis rice(Bt rice,carrying crylAb gene) on the rhizosphere bacterial flora under paddy field conditions,the community structure and its quantitative variations of bacteria in different rhizosphere areas(root surface,the rhizosphere,inner root) of two Bt rice lines(KMD1 and KMD2) and the parent XiuShui11(XS11) under different developmental stages were compared.For three lines,the average bacteria number reached the peak at shooting stage and the bottom at heading stage.The average bacteria number in the root surface and inner root,but not in the rhizosphere,of KMD1 or KMD2 was significantly higher than that of XS11.Twenty genera were classified from 303 strains.The strains isolated from three lines mostly belonged to same genus,but the bacteria number were different,in which the quantities of Staphylococcus,Corynebacterium and Agromyces of two Bt rice lines were higher than those of XS11,whereas Trichococcus of two Bt rice lines were less than that of XS11.There were no significant differences of Shannon-wiener、Simpson and Pielou indexes for the total bacterial community among three lines.This work demonstrated that Bt rice would lead to the quantitative variations of bacteria in rice soil rhizosphere,dependent on the rice developmental stages and rhizosphere areas.

关 键 词：BT水稻 根际 细菌区系 群落多样性 

分 类 号：S511[农业科学—作物学] S154.3