补肾活血方提高妊娠大鼠胚泡着床率的实验研究  

Study of Bushen Huoxue Prescription on increasing blastocyst nidation rate in pregnancy mice

作　　者：王雅楠[1] 宋殿荣[2] 刘亚琴[1] 王瑞[1] 张崴[2] 

机构地区：[1]天津中医药大学,研究生天津300193 [2]天津中医药大学第二附属医院,天津300150

出　　处：《天津中医药》2009年第3期233-235,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市高等学校科技发展基金项目(编号:20050301)

摘　　要：[目的]观察中药补肾活血方对妊娠大鼠胚泡着床率的影响。[方法]选用妊娠SD大鼠随机分为空白组、模型组和中药低、中、高剂量组,于妊娠第5天扫描电镜观察各组子宫内膜胞饮突发育情况,妊娠第10天计数各组大鼠胚泡着床数。[结果]模型组子宫内膜表面未见明显的胞饮突发育,表现为增生早期的子宫内膜变化,中药中、高剂量组可见发育中的胞饮突,发育形态明显超前于模型组,接近于空白组;模型组胚泡着床数显著低于空白组(P<0.01),中药中、高剂量组胚泡着床数显著高于模型组(P<0.01)。[结论]临床用量的补肾活血方能明显改善大鼠子宫内膜表面胞饮突的发育,有助于子宫内膜容受性的建立,从而最终提高胚泡的着床率。[Objective] To observe the effect of Bushen Huoxue prescription on blastocyst nidation in pregnancy mice. [Methods] SD pregnancy mice were randomly divided into control group, model group, low-dose treat group （L-D）, mide-dose treat group （M-D） and high-dose treat group （H-D）. On the fifth day of pregnancy （Pd5）, development of endometrial pinopodes was determined by scanning electron microscopy. On Pd10, the numbers of implanted blastocyst was counted. [Results] Developing pinopodes of endometrial surface were bad in model group as endometrial of early proliferative phase. Developed pinopodes, however, were observed in M-D and H-D treat group compared with control group. The number of implanted blastocysts in model group was significantly lower than that of control group （P〈0.01）, and M-D and H-D treat group were higher than model group （P〈0.01）. [Conclusion] It is suggested that Bushen Huoxue pre- scription can improve the pinopodes developing in endometrial surface helped to eventually the establishment of reception of the endometria and improve the blastocyst implantation rate.

关 键 词：补肾活血方 胞饮突 子宫内膜容受性 

分 类 号：R285.5[医药卫生—中药学]