纳米TiO2/丝素蛋白多孔膜的体外降解性能  

Preparation and In-vitro Degradation of Nano-TiO_2/Silk Fibroin Porous Films

作　　者：郑敏[1] 严自力[2] 沈锦玉[1] 朱海霖[1] 冯新星[1] 陈建勇[1] 

机构地区：[1]浙江理工大学材料与纺织学院,先进纺织材料与制备技术教育部重点实验室,杭州310018 [2]总后军需装备研究所军用汉麻材料研究中心,北京100088

出　　处：《蚕业科学》2009年第2期422-426,共5页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(编号50573068);浙江省自然科学基金项目(编号Y407295);国家重大基础性研究发展计划“973”前期研究专项课题(编号2008CB617506);长江学者和创新团队发展计划项目(编号IRT0654)

摘　　要：为改善丝素蛋白膜的生物活性功能,通过冷冻干燥的方法制备了纳米TiO2/丝素蛋白多孔膜,并在人体模拟体液（simulated body fluid,SBF）中进行了体外降解试验。测试结果表明：纳米TiO2/丝素蛋白多孔膜具有较好的平均孔径和孔隙率,分别为22-53μm和86%-92%;降解20 d后,孔洞界限逐渐被破坏,复合多孔膜的失重率增大到35%以上;降解后多孔膜的结晶度有大于降解前的趋势;在多孔膜的内壁有羟基磷灰石的生成,具有很好的生物活性。据此认为,采用冷冻干燥法制备的纳米TiO2/丝素蛋白多孔膜,有望进一步开发成为软骨替代材料或创面涂敷材料。In this paper, the Nano-TiO2/silk fibroin porous films were prepared using the freeze-drying method in order to improve the biological activity of silk fibroin films. The in vitro degradation of these films in human simulated body fluid was investigated. The results indicated that the Nano-TiO2/silk fibroin porous films had better average pore diameter （22 - 53 μm） and porosity （86% - 92%）. After 20 days of degradation, the edge of pores was destroyed with the increased weightlessness of films, and the weight loss was over 35%. It was observed that the crystallinity of films had the tendency to become higher after the degradation. These porous films showed good biological activity as the formation of hydroxyapatite occurred in the inner walls of the porous films. It is thus suggested that the Nano- TiO2/silk fibroin porous films prepared through freeze-drying method is a potential substitution material for cartilage or for coating wounds.

关 键 词：丝素蛋白 纳米TIO2 多孔膜 人体模拟体液 降解 

分 类 号：R318.08[医药卫生—生物医学工程]