心肌Na^+,K^+-ATP酶在抑郁症时的活性及mRNA表达  

Myocardium Na^+,K^+-ATPase activity and its mRNA expression in depression in rats

作　　者：李素琴[1] 马欣欣[1] 李晓晶[1] 卢振敏[1] 师贵文[1] 刘玉欣[1] 董彬[1] 刘杰[2] 胡景荣[2] 孙东[2] 

机构地区：[1]河北省疾病预防控制中心,石家庄市050021 [2]河北省精神卫生中心

出　　处：《河北医药》2009年第12期1422-1424,共3页Hebei Medical Journal

摘　　要：目的探讨Na+,K+-ATP酶在抑郁症发病中的作用及其和单胺类神经递质之间的关系。方法对SD大鼠进行慢性随机刺激建立抑郁症模型,检测大鼠心肌Na+,K+-ATP酶的活性及mRNA表达,海马组织及血液中去甲肾上腺素(NA)和5-羟色胺代谢物5羟-吲哚乙酸(5-H IAA)水平。结果抑郁症大鼠心肌Na+,K+-ATP酶的转录水平下调(P<0.01);酶的活性明显降低(P<0.01);海马及血浆中的NA及5-H IAA水平显著下降(P<0.01)。结论推测抑郁症时神经体液因素的变化可能是引起心肌细胞Na+,K+-ATP酶分子变化的原因之一,该酶在抑郁症的发病机制中有一定的生理和病理意义。Objective To investigate the effect of Na^＋,K^＋-ATP ase in depression and the relationship between Na^＋,K^＋-ATP ase and monoamine neurotransmitters in rats. Methods The depression models of SD rats were established by chronic stimulation randomly for 21 days. The mRNA expression of myocardiumNa^＋,K^＋-ATP ase, the enzyme activity, norepinephrine （NA） and 5-hydroxyindoleacetic acid （5-HIAA, the metabolic product of serotonin）that participated in the regulation of affection, sleeping and memory, were detected. Results The transcriptional levels of Na^＋,K^＋-ATP ase were decreased in depression models of rats. The activity of Na^＋,K^＋-ATP ase was decreased in rat depression model. NA and 5-HIAA contents in hippocampus were significant decreased in depression rats. Conclusion The change of nerrohumor factors may be one of the primary causes for the change of myocardium Na^＋,K^＋-ATP ase in depression. The enzyme may be of physiological and pathologic significance in pathogenesy of depression.

关 键 词：Na+ K+-ATP酶 酶活性 MRNA表达 抑郁症 海马 神经递质 

分 类 号：R318[医药卫生—生物医学工程] Q463[医药卫生—基础医学]