龙胆石斑鱼源美人鱼发光杆菌的生物学特性与系统发育学分析  

Biological characterization and phylogenetic analysis of Photobacterium damselae subsp.damselae from diseased Epinephelus lanceolatus L.

作　　者：张晓君[1] 秦国民[1] 陈翠珍[2] 房海[2] 阎斌伦[1] 

机构地区：[1]淮海工学院海洋学院江苏省海洋生物技术重点建设实验室,连云港222005 [2]河北科技师范学院动物科学系,秦皇岛066600

出　　处：《渔业科学进展》2009年第3期38-43,共6页Progress in Fishery Sciences

基　　金：淮海工学院引进人才科研启动基金项目(KQ07106);连云港市科技攻关项目(CN0826)共同资助

摘　　要：对从1尾病死的观赏用龙胆石斑鱼Epinephelus lanceolatus L.中分离到的细菌,进行了形态特征、理化特性和对抗菌类药物的敏感性等较系统的表观生物学性状鉴定。同时,测定了16S rRNA基因序列、分析了相关细菌相应序列的同源性、构建了系统发生树。结果表明,供试两株纯培养菌(编号:HQ061227-1、HQ061227-2)为发光杆菌属Photobacterium(Beijerinck 1889)的美人鱼发光杆菌美人鱼亚种P.damselae subsp.damselae(Love et al.1982;Smith et al.1991),用HQ061227-1株作为代表菌株的16S rRNA基因序列长度(不包括引物结合区)为1469bp(GenBank登录号:EF635307),与GenBank数据库中美人鱼发光杆菌美人鱼亚种的同源性在99%。药敏试验结果显示,对供试37种抗菌药物中的青霉素G等4种耐药,对头孢唑啉等32种敏感,对氨苄青霉素低敏。The biological characteristics and phylogenetic analysis of bacteria isolated from Epinephelus lanceolatus L. were studied, including morphological characteristics, physiological and biochemical characteristics. In addition, the 16S rRNA gene were partially sequenced and compared with sequences registered in databases; and molecular phylogenetic trees were constructed. The results showed that the two pure strains （HQ061227-1,HQ061227-2）belonged to P. damselae subsp, damselae （Love et al. 1982 ; Smith et al. 1991） of Photobacterium （Beijerinck 1889 ）, the sequenced 16S rRNA gene of strain HQ061227-1（accession No. EF635307） is 1 469bp in length, and exhibited high similarity （99%） with the 16S rRNA gene of P. damselae from GenBank database. Antibiotic sensitivity using 37 antimicrobial agents showed that the isolates were sensitive to 32 agents including cefazolin, resistant to 4 agents including penicillin G, and slightly sensitive to ampicillin.

关 键 词：龙胆石斑鱼 美人鱼发光杆菌美人鱼亚种 生物学特性 系统发育学 

分 类 号：S943[农业科学—水产养殖]