黄姑鱼群体遗传结构的同工酶分析  

Genetic structures of Nibea albiflora as revealed by isozyme analysis

作　　者：韩志强[1,2] 高天翔[1] 韩冰[1] 庄志猛[2] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,青岛266003 [2]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《渔业科学进展》2009年第3期44-48,共5页Progress in Fishery Sciences

基　　金：国家自然科学基金(30471329);国家重点基础研究发展计划项目(2005CB422306);国家高新技术发展计划项目(2006AA09Z418)共同资助

摘　　要：采用水平淀粉凝胶电泳技术对黄姑鱼青岛、舟山和厦门3个群体的遗传结构和遗传分化进行了研究。在G3PDH、IDHP、LDH、MDH、PGDH、PGM、SDH和SOD等8种酶中,共检测到11个基因位点。3个黄姑鱼群体在G3PDH*和PGM*位点上均呈多态,其多态位点比例均为0.2。3个群体的平均杂合度观测值和预期值分别在0.0101～0.0379和0.0234～0.0399之间,平均每个位点的有效等位基因数在1.0290～1.0528之间。3个群体间遗传距离和遗传一致度分别为0.0004～0.0008和0.9992～0.9996。研究结果表明,3个黄姑鱼群体之间无明显的遗传分化。Horizontal starch gel electrophoresis was used to investigate the genetic structure and differentiation of the three populations （Qingdao, Zhoushan and Xiamen） of Nibea albiflora. Eleven putative loci were revealed by eight enzymes （G3PDH, IDHP, LDH, MDH, PGDH, PGM, SDH and SOD）. The percentage of polymorphic loci for each population was 0. 2. The mean observed and expected heterozygosity ranged from 0. 010 1 to 0. 037 9 and from 0. 023 4 to 0. 039 9, respectively. The average number of efficient allele for each locus varied from 1. 029 0 to 1. 052 8. The genetic distances among populations were from 0. 000 4 to 0. 000 8, and the genetic similarities were from 0. 999 2 to 0. 999 6. The results showed that there was no significant genetic differentiation among the three populations of N. albiflora.

关 键 词：黄姑鱼 同工酶 遗传变异 

分 类 号：Q55[生物学—生物化学]