柳树杂交子代AFLP的分子鉴别与多态性分析  

Analysis On Molecular Identification and Polymorphism of Hybrid Progenies in Salix by AFLP Technique

作　　者：李文清[1] 解孝满 刘启虎[1] 徐金光 解荷锋 王利[3] 彭广伟 邢世岩[3] 陈立亭 

机构地区：[1]山东省林木种苗繁育中心,济南250014 [2]山东省林木种苗站 [3]山东农业大学林学院 [4]新汶园林所

出　　处：《山东林业科技》2009年第3期63-64,72,共3页Journal of Shandong Forestry Science and Technology

基　　金：山东省农业良种工程课题(编号:200599和200690);山东省林木种质资源库建设项目(一期)

摘　　要：利用筛选出的5对引物,对来自菏泽的2号、8号、172号、SH4、SH7、SH9、SH10、SH15、SH17、SH18、SH22、SH25、SH26、SH28、SH31、SH32、SH37、SH39、SH48、SH49共20个柳树种质进行了AFLP分子鉴别和多态性分析研究。结果表明:5对引物共产生645条谱带,多态带542条,多态带的比例为84.03%;每对引物鉴别效率为100%。AFLP是进行鉴别亲缘关系比较近的柳树种质的有效方法。We screened out 5 pairs of primers to study the molecular identification and polymorphism among ‘2＇,‘8＇ ‘172’, ‘SH4＇, ‘SH7＇,‘SH9＇,‘SH10＇, ‘SH15＇,‘SH17＇, ‘SH18＇, ‘SH22＇,‘SH25＇, ‘SH26＇,‘SH28＇,‘SH31＇, ‘SH32’,‘SH37＇,‘SH39’,‘SH48’ and ‘SH49＇ Salix hybrid progenies from Heze city, Shandong province by amplified fragment length polymorphism（AFLP）technique. The results show that 5 pairs of primers produce totally 645 bands, 542 polymorphic bands. The polymorphic bands account for 84.03% and the identification efficiency of each pair of primers is 100%. AFLP is an effective method for identifying Salix germplasms with closer genetic relationship.

关 键 词：柳树 种质 多态性 AFLP 

分 类 号：S792.12[农业科学—林木遗传育种]