外源亚精胺对模拟干旱胁迫下番茄幼苗活性氧水平和抗氧化系统的影响  

Effects of Exogenous Spermidine on Reactive Oxygen Levels and Antioxidative System of Tomato Seedling under Polyethlene Glycol Stress

作　　者：张春梅[1,2] 邹志荣[1] 张志新[1] 黄志[1] 

机构地区：[1]西北农林科技大学园艺学院,杨凌712100 [2]河西学院农学系,张掖734000

出　　处：《应用与环境生物学报》2009年第3期301-307,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家“十一五”攻关项目(No. 2007BAD79B04)~~

摘　　要：以耐旱性不同的2个品种番茄(‘毛粉802’和‘皇冠’)为试材,采用营养液栽培,研究了聚乙二醇(PEG)模拟的干旱胁迫下喷施外源亚精胺(Spd)对番茄幼苗活性氧(ROS)水平和抗氧化系统的影响.结果表明,喷施0.1mmolL-1Spd降低了7.5%PEG胁迫下2个品种番茄幼苗叶中超氧阴离子(O2-·)产生速率、过氧化氢(H2O2)、丙二醛(MDA)含量的上升,增加了超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)以及AsA-GSH循环中抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)的活性,提高了抗氧化物质抗坏血酸(AsA)、还原型谷胱甘肽(GSH)的含量,对单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)活性没有影响.耐旱性较强的‘毛粉802’幼苗叶中抗氧化酶活性和抗氧化剂升高幅度明显大于耐旱性较弱的‘皇冠’,ROS积累速度和膜脂过氧化程度相对较低,喷施Spd处理对‘皇冠’效果更为明显.这表明外源Spd通过减少干旱胁迫下番茄幼苗体内ROS的产生,提高植株体内抗氧化系统中抗氧化酶活性、抗氧化剂含量和降低膜脂过氧化水平,起到缓解干旱胁迫对番茄幼苗伤害的作用,增强幼苗对干旱逆境的适应性.Two different drought-tolerant tomato cultivars were investigated under drought stress induced by 7.5% polyethylene glycol （PEG6000） in a nutrient solution culture system. The results showed that exogenous spermidine （Spd） （0.1 mmol L^-1 ） treatment decreased O2^-· production rate, and MDA and H2O2 contents, raised SOD, POD, CAT, APX and GR activities in the seedlings, and had no effect on MDHAR and DHAR activities. Spd treatment of ‘Huangguan＇ seedlings with lower drought-resistance was more effective than that of ‘Maofen No. 802＇ seedlings with stronger drought-resistance. It indicated that exogenous Spd treatment could to enhance the activities of some antioxidant enzymes and the contents of antioxidant substances, restrain embrane lipid peroxidation, reduce ROS levels and finally enhance the tolerance of tomato seedlings to drought environments.

关 键 词：亚精胺 番茄 干旱胁迫 活性氧 抗氧化系统 

分 类 号：S641.2[农业科学—蔬菜学]