草鱼肠道纤维素酶产生菌主要种类的分离与鉴定  

Isolation and Identification of Major Cellulase-producing Fungi in Intestines of Grass Carp

作　　者：何力[1] 郝勃[2] 谢从新[2] 罗晓松[1] 张征[1] 朱祥云[1] 

机构地区：[1]中国水产科学研究院长江水产研究所,荆州434000 [2]华中农业大学水产学院,武汉430070

出　　处：《应用与环境生物学报》2009年第3期414-418,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技支撑计划项目(No.2006BA D01A12);中国水产科学院淡水生态与健康养殖重点实验室开放课题项目(No.FEA-2006108)~~

摘　　要：从我国特有的草鱼肠道内分离到纤维素酶产生菌,其中4株具有较高的纤维素分解能力,测量了其羧甲基纤维素酶(CMCase)、棉花酶和滤纸酶(FPase)活性,并进行了种类鉴定.结果表明,草鱼肠道具有一定的纤维素分解能力,可能来自于肠道细菌所产生的外源性纤维素酶的协同作用;草鱼肠道内纤维素分解菌具有较高酶活性;不同菌株所产生的相同纤维素酶的活性存在差异;其中,X7的CMCase活性最高,达0.83U/mL,X5的棉花酶活性最高,达0.87U/mL,X8的FPase活性最高,达0.54U/mL.4种菌株的菌落形态有差异,经16S rRNA基因鉴定,X5、X6、X7为枯草芽胞杆菌(Bacillus subtilis),X8为阿氏肠杆菌(Enterobacter asburiae).Cellulase producing strains were isolated from the intestines of grass carp which is endemic to China, and 4 of them were found with high cellulase activity. The activities ofcarboxymethyl cellulase （CMCase）, cotton lyase and filter paper enzyme （FPase） were measured and the species were identified. The results showed that the intestines of grass carp had the ability to decompose cellulase, which might be the synergistic effect of exogenous cellulase produced in the intestines. The cellulase producing strains in grass carp intestines possessed high enzyme activities, which were different in the strains. CMCase in X7 had the higest activity with the value of 0.83 U/mL, while X5 and X8 had the highest cotton lyase and FPase with the values of 0.87 and 0.54 U/mL, respectively. The colony morphology was different among the 4 strains. 16S rRNA genotype identification indicated that X5, X6 and X7 belonged to Bacillus subtilis and X8 to Enterobaeter asburiae.

关 键 词：草鱼 肠道 纤维素酶产生菌 分离 鉴定 

分 类 号：Q93[生物学—微生物学]