DNA Copy Number Aberrations in Breast Cancer by Array Comparative Genomic Hybridization  

作　　者：Jian Li Kai Wang Shengting Li Vera Timmermans-Wielenga Fritz Rank Carsten Wiuf Xiuqing Zhang Huanming Yang Lars Bolund 

机构地区：[1]Institute of Human Genetics, University ofAarhus, DK-8000, Aarhus, Denmark [2]Beijing Genomics Institute at Shenzhen, Shenzhen 518083, China [3]Bioinformatics Research Center, University of Aarhus, DK-8000, Aarhus, Denmark [4]Department of Pathology, Center of Diagnostic Investigations, DK-2100, Copenhagen, Denmark [5]Danish Center for Translational Breast Cancer Research, DK-2100, Copenhagen, Denmark

出　　处：《Genomics, Proteomics & Bioinformatics》2009年第1期13-24,共12页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the Danish Cancer Soci-ety through the budget of the Institute of Cancer Biol-ogy and by grants from the Danish Medical Research Council, the Natural and Medical Sciences Committee of the Danish Cancer Society, Novo Nordisk, the John and Birthe Meyer Foundation, the Solar Fonden, the Stensbygaard Fonden, the Kai Langeog Gundhild Kai Lange Fond, the will of Edith Stern, and the "Race Against Breast Cancer" Project;The support of the Marketing Department at the Danish Cancer Society is greatly appreciated;supported by a project grant from the Hi-Tech Research and Development Program of China (2006AA02A301)

摘　　要：Array comparative genomic hybridization （CGH） has been popularly used for analyzing DNA copy number variations in diseases like cancer. In this study, we investigated 82 sporadic samples from 49 breast cancer patients using 1-Mb resolution bacterial artificial chromosome （2GH arrays. A number of highly frequent genomic aberrations were discovered, which may act as ＂drivers＂ of tumor progression. Meanwhile, the genomic profiles of four ＂normal＂ breast tissue samples taken at least 2 cm away from the primary tumor sites were also found to have some genomic aberrations that recurred with high frequency in the primary tumors, which may have important implications for clinical therapy. Additionally, we performed class comparison and class prediction for various clinicopathological parameters, and a list of characteristic genomic aberrations associated with different clinicopathological phenotypes was compiled. Our study provides clues for further investigations of the underlying mechanisms of breast carcinogenesis.Array comparative genomic hybridization （CGH） has been popularly used for analyzing DNA copy number variations in diseases like cancer. In this study, we investigated 82 sporadic samples from 49 breast cancer patients using 1-Mb resolution bacterial artificial chromosome （2GH arrays. A number of highly frequent genomic aberrations were discovered, which may act as ＂drivers＂ of tumor progression. Meanwhile, the genomic profiles of four ＂normal＂ breast tissue samples taken at least 2 cm away from the primary tumor sites were also found to have some genomic aberrations that recurred with high frequency in the primary tumors, which may have important implications for clinical therapy. Additionally, we performed class comparison and class prediction for various clinicopathological parameters, and a list of characteristic genomic aberrations associated with different clinicopathological phenotypes was compiled. Our study provides clues for further investigations of the underlying mechanisms of breast carcinogenesis.

关 键 词：breast cancer genomic aberration array CGH clinicopathological parameter 

分 类 号：Q78[生物学—分子生物学] S858.292[农业科学—临床兽医学]