钙离子对坐骨神经腓肠肌神经肌肉接头神经递质释放的作用  

The research on the effect of Ca^(2+) in sciatic nerve's synaptic vesicles by detecting reactions of gastrocnemius

作　　者：王宏[1] 李红芳[2] 陈方[1] 赵润平[1] 王成祥[1] 顾维刚[1] 韩杰[1] 

机构地区：[1]兰州大学第一临床医学院,兰州730000 [2]兰州大学基础医学院,兰州730000

出　　处：《白求恩军医学院学报》2009年第3期143-145,共3页Journal of Bethune Military Medical College

基　　金：教育部"国家大学生创新性实验计划"资助(项目编码:071073040)

摘　　要：目的探讨神经突触小体中神经递质量子释放的机制,并提出假设予以验证,从而明确Ca2+在这一过程中的作用。方法应用钙通道阻滞剂异搏定、蛋白激酶A的阻滞H-89选择性作用于分离的蟾蜍坐骨神经腓肠肌标本上,通过观察腓肠肌的收缩程度,验证实验假设机制。结果Ca2+组收缩曲线的振幅较正常组收缩曲线的振幅大(P<0.05);异搏定组收缩曲线的振幅较正常组收缩曲线的振幅小(P<0.05);H-89组收缩曲线的振幅较正常组收缩曲线的振幅小(P<0.05);Mg2+组收缩曲线的振幅较正常组收缩曲线的振幅小(P<0.05)。结论Ca2+促进了突触小体中神经递质的释放;异搏定、H-89、Mg2+抑制了突触小体中神经递质的释放。Objective To explore the molecular mechanisms underlying neurotransmitter releasing from synaptic vesicles and confirm the exact effect in the process by proposing relative assumptions molecular mechanisms. Methods By using the verapamil （the antagonist against Ca^2＋ ion channel） and H-89 （the antagonist against PKA） to work selectively on the toads＇ isolated sciatic nerve, we could confirm the assumption in the consequence of observations on how much the muscle shrink after being stimulated. Results The amplitude of Ca^2＋ group＇s shrinkage curve was larger than the control group＇s（ P 〈0.05） ; The amplitude of the verapamil group is smaller than the one of control group＇s （ P 〈0.05）. The amplitude of the H-89 group was smaller than that of the control group （ P 〈 0.05 ）. The amplitude of the Mg^2＋ group was smaller than that of the control group （ P 〈 0.05 ）. Conclusion Ca^2＋ can stimulate neurotransmitter to release from synaptic vesicles. In the process of releasing, verapamil, H-89 and Mg^2＋ play negative roles which inhibit neurotransmitter releasing.

关 键 词：CA2+ SNARE核心复合物 神经递质释放机制 蛋白激酶A 突触 

分 类 号：R338[医药卫生—人体生理学]