白藜芦醇诱导白血病KG-1细胞凋亡及对其细胞周期的影响  

The apoptosis effect of leukemia KG-1 cells induced by resveratrol and its effect on cell cycle

作　　者：徐红俊[1] 

机构地区：[1]石家庄卫生学校,河北石家庄050200

出　　处：《中国医药导报》2009年第19期21-22,共2页China Medical Herald

摘　　要：目的:探讨白藜芦醇诱导人白血病细胞株KG-1细胞凋亡的作用。方法:以不同浓度的白藜芦醇作用KG-1细胞,用噻唑蓝(MTT)比色法分析细胞生长状态;透射电镜观察KG-1细胞凋亡的形态学变化;流式细胞术(FMC)测定细胞凋亡率和细胞周期分布。结果:白藜芦醇可明显抑制KG-1细胞增殖(P<0.05),与对照组比较,白藜芦醇作用后,KG-1细胞发生S期阻滞(P<0.01),且细胞凋亡率增高(P<0.01)。结论:白藜芦醇能明显抑制KG-1细胞增殖,使细胞周期阻滞在S期,并能进一步诱导其凋亡。Objective： To investigate human leukemia KG-1 ceils apoptosis induced by resveratrol. Methods： After treated with different concentrations RES,the suppressive effect of RES on proliferation of KG-1 ceils was analyzed by MTT method. Transmission electron microscope technique were used to detect the apoptosis status of KG-1 cells.The cell cycle and apoptosis rate of KG-1 cells treated with RES were detected by flow cytometry. Results： RES could obviously inhibit proliferation of KG-1 ceils （P〈0.05）.Compared with the control group, after treated with RES, the cells number in S phase of KG-1 cells increased（P〈0.01）, and the apoptosis rate increased（P〈0.01）. Conclusion： RES could significantly inhibit the proliferation of KG-1 cells and block the cell cycle at S. And in the end RES could induce the apoptosis of KG-1 cells.

关 键 词：白藜芦醇 KG-1细胞 细胞凋亡 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]