不同穴位电针对大鼠脊髓损伤植入脐血干细胞后生长相关蛋白-43、髓磷脂碱性蛋白表达的影响  

Expression of GAP-43,MBP by Electrical Stimulation in Different Sites of Spinal Cord Injured Rats after Human Umbilical Cord Blood Stem Cells Transplantation

作　　者：孙迎春[1,2] 李建军[1,2] 高莉敏[3] 高峰[1,2] 刘舒佳[1,2] 季凤清[4] 

机构地区：[1]首都医科大学康复医学院,北京市100068 [2]中国康复研究中心北京博爱医院,北京市100068 [3]北京中医药大学管理学院,北京市100029 [4]首都医科大学,北京市100069

出　　处：《中国康复理论与实践》2009年第6期524-528,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：中国博士后科学基金面上项目(20070420405);中国康复研究中心科研基金(2007-19)

摘　　要：目的探讨不同电针对大鼠脊髓损伤植入脐血干细胞后生长相关蛋白(GAP)-43、髓磷脂碱性蛋白(MBP)表达的影响。方法将192只清洁级SD大鼠用NYU打击器采用Allen's法制成T10-11段脊髓损伤模型,造模成功后查随机表分为:①A组:运动区头皮表面投影区电刺激组,又分为A1组(电刺激+脐血干细胞移植)和A2组(只进行电刺激);②B组:损伤局部电刺激组,又分为B1组(电刺激+脐血干细胞移植)和B2组(只进行电刺激);③C组:运动区头皮表面投影区电刺激+局部电刺激组,又分为C1组(电刺激+脐血干细胞移植)和C2组(只进行电刺激);④D组:损伤模型组,以分为D1组(移植脐血干细胞不进行电刺激)和D2组(不移植不进行电刺激)。各组分别于1、2、3、4、8、12周时取材,采用生物素葡聚糖胺(BDA)皮质脊髓束顺行示踪,观察损伤区残存或再生的神经纤维的分布情况;通过免疫组织化学荧光染色检测GAP-43、MBP及MAB1281抗人核抗体的阳性表达。结果①NYU是一种用于Allen's法制备大鼠脊髓撞击损伤模型的撞击器,具有可使撞击势能量化的特点,获得的模型稳定、可重复性好、成功率高。②在本组研究各时间点上,电刺激对干细胞移植后微环境中GAP-43、MBP水平均有显著的正向干预作用,均较单纯电刺激有显著的正向协同干预作用,头针加体针的影响大于单纯头针或体针。③在本组研究各时间点上,电刺激对损伤后GAP-43、MBP水平均有显著的正向干预作用,不依赖于是否进行干细胞移植。结论①干细胞移植后,微环境的变化朝有利于神经功能恢复方向发展,说明干细胞移植能促进神经功能恢复以及本研究中干细胞移植成功。②电刺激对干细胞移植后微环境中GAP-43、MBP有显著的正向干预作用;头针加体针的影响优于单纯头针或体针;电刺激对损伤后微环境显著正向干预作用是独立的,不依赖于是否进行干细胞移植;电刺激�Objective To explore the application values of effect on the expression of growth associated protein-43 （GAP-43） and myelin basic protein（MBP） by electrical stimulation in different sites of spinal cord injured rats after the human umbilical cord blood stem cells transplantation. Methods In this study the subjects adopted are clean grade rats, which were performed with Allen＇s method by NYU blow device, resulting in SCI models. 192 rats successfully modeled were divided into groups according to the random table. Group A received electric stimulation in the scalp surface projection area of motor area： Group A1 received electric stimulation and transplantation of umbilical cord blood stem cell, while Group A2 only received electric stimulation; Group B received local electric stimulation at damaged site： Group B1 received electric stimulation and transplantation of umbilical cord blood stem cell, while Group B2 only received electric stimulation; Group C received electric stimulation in the scalp surface projection area of motor area and local electric stimulation at damaged site： Group C1 received electric stimulation and transplantation of umbilical cord blood stem cell, while Group C2 only received electric stimulation; Group D are just the SCI models： Group D1 received transplantation of umbilical cord blood stem cell without electric stimulation, while Group D2 neither received cell transplantation nor electric stimulation. The sacrifice time of rats was the 1st week, 2nd week, 3rd week, 4th week, 8th week, and 12th week after operation respectively. The distribution of the remained or regenerative neurons at damaged areas by corticospinal tract anterograde tracer with Biotinylated dextran amine （BDA）, and we also detected the content changes of GAP-43, MBP and the MAB1281 specific expression of the human nucleus of human umbilical cord blood stem cells. Results 1）. NYU is a blow device for Allen＇s rat model of SCI, which could quantify the crash potential energy, and gain a stable an

关 键 词：针灸 干细胞移植 脊髓损伤 生长相关蛋白(GAP-43) 髓磷脂碱性蛋白(MBP) 

分 类 号：R651.2[医药卫生—外科学]