Polycomb基因家族在大鼠精子发生过程中的表达  

Expression of Polycomb-Group Gene during Rat Spermatogenesis

作　　者：汪卓群[1,2] 鲁爽[1,2] 金孝华[1,2] 贾孟春[2] 石心泉[2] 马旭[2] 

机构地区：[1]北京协和医学院研究生院,北京100085 [2]国家人口计生委科学技术研究所,北京100081

出　　处：《中国实验动物学报》2009年第3期172-175,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家“973”基础研究基金(编号:2007CB511905)

摘　　要：目的检测大鼠精子发生不同阶段细胞中Polycomb-group(Pc-G)家族在mRNA水平上表达是否有差异。方法提纯大鼠精子发生过程中的精原细胞、精母细胞、圆形精子细胞以及支持细胞,用荧光定量PCR方法检测Pc-G家族基因mRNA表达量。结果Pc-G基因家族中Ezh2、Eed、Bmi-1在精子发生中后期高表达;在各生精细胞中,YY1基因表达量低于支持细胞。结论Pc-G基因家族在精子发生各阶段细胞中特征性表达,与精子发生具有相关性,可能对精子发生分化和维持遗传稳定性都有重要的作用。Objective To assess whether polycomb-group （Pc-G） mRNA levels change during differentiation of rat sperm cells. Methods Real-time PCR method was used to detect the relative mRNA levels of Pc-G genes originating from spermtogonia, spermoocyte, round sperm and sertoli cells during rat spermatogenesis. Results Pe-G genes Ezh2, Eed and Bmi-1 were highly expressed at the later stage of spermatogenesis. However, mRNA level of YYl in spermatogenic cells was lower than that in Sertoli cells. Conclusion Pc-G genes are expressed in spermatogenic cells and correlated with spermatogenesis indicates that Pc-G genes may play an important role in directing sperm cells differentiation and maintaining the genetic stability.

关 键 词：Polycomb基因家族 精子发生 

分 类 号：R33[医药卫生—人体生理学]