基质金属蛋白酶及其组织抑制剂在大鼠慢性高眼压模型筛板区巩膜组织中的表达  

Expression of matrix metalloproteinases and inhibitors on the scleral tissue of lamina cribrosa in rat with experimental chronic ocular hypertension

作　　者：徐韶琳[1] 高志卓[1] 王英[1] 陈杰[1] 

机构地区：[1]吉林大学第二医院眼科,长春130041

出　　处：《中华眼科杂志》2009年第3期260-265,共6页Chinese Journal of Ophthalmology

基　　金：吉林省科技厅科学基金（200505114）

摘　　要：目的探讨大鼠慢性高眼压模型筛板区巩膜组织中基质金属蛋白酶（MMP）及其抑制剂（TIMP）的表达与青光眼发病机制的关系。方法实验研究。将43只wistar大鼠的左眼成功地建立为慢性高眼压模型，对侧有眼为对照组。采用计算机随机数字表法将实验大鼠分为3组，分别应用免疫组织化学法（13只鼠）、免疫印迹法（15只鼠）及逆转录聚合酶链反应（RT—PCR）法（15只鼠）检测大鼠筛板区巩膜组织中MMP-2、MMP-9、TIMP-1、TIMP-2的表达情况。应用SAS统计学软件，对免疫印迹法和RT—PCR法检测MMP-2、MMP-9、TIMP-1、TIMP-2表达的4个定量指标，分别采用具有一个重复测量的两因素设计定量资料的方差分析，以P〈0．01作为差异有统计学意义。结果（1）免疫组织化学检测结果：实验眼筛板区巩膜组织中细胞核和细胞浆中MMP-2、MMP-9表达明显，对照眼均未见MMP-2、MMP-9表达；实验眼和对照眼筛板区巩膜组织中细胞核和细胞浆中均未见TIMP—1、TTMP-2表达。（2）免疫印迹法检测结果：平均灰度值：实验眼的MMP-2为193．88±8．84，MMP-9为202．65±8．37，TIMP-1为283．63±5．65，TIMP-2为284．75±5．50；对照眼的MMP-2为117．38±10．76，MMP-9为134．13±5．06，TIMP-1为186．88±7．14，TIMP-2为183．0±5．58。（3）RT—PCR法检测结果：平均灰度值：实验服的MMP-2为200．50±3．25，MMP-9为200．13±2．95，TIMP—1为201．88±3．14，TIMP-2为195．50±3．55；对照眼的MMP—2为181．88±9．36，MMP-9为181．75±5．85，TIMP-1为179．25±9．21，TIMP-2为179．75±7．12。实验眼与对照眼的表达差异有统计学意义（MMP-2：F=405．55，MMP-9：F=436．11，TIMP-1：F=1167．77，TIMP-2：，=4629．64；均P〈0．01）。结论大鼠慢性高眼压模型筛板区巩膜组织中MMP-2、MMP-9、TIMP-1、TIMP-2的表达明显增加。Objective To investigate the expression of matrix metalloproteinases（MMPs）and tissue inhibitors of metalloproteinases（TIMPs） in the sclera of lamina cribrosa in rat chronic elevated intraocular pressure （IOP） and its the relationship with the pathogenesis of glaucoma. Methods It was an experimental study. Chronic ocular hypertension （OHT） model was induced in the left eyes of 43 Wistar rats by cauterizing episeleral venous while the right eyes were used as control. The rats were divided into three groups： 13 for immunohistology, 15 for Western blotting, and 15 for RT-PCR by randomly computerized assignment and sacrificed at week 12 after OHT induction. The expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the sclera lamina cribrosa was detected using immunohistology, Western blotting, and RT- PCR technique, respectively. Repeated measures ANOVA of two-factor Studies in SAS statistic software were used to analyze the data. Results After 12 weeks, compared with control eyes, the IOP of rat OHT eyes was significantly （F = 1519.67 ,P 〈0.01 ） elevated. The expression of MMP-2 and MMP-9 was increased in cell nucleus and cytoplasm in sclera lamina cribrosa in rat OHT when compared to control eyes with negative expression. No expressions of TIMP-1 ,TIMP-2 in sclera lamina cribrosa were found in both OHT and control eyes. The mean gray-scale values of MMP-2, MMP-9, TIMP-1, and TIMP-2 in OHT were significantly （ F = 405.55, F = 436.11 ,F = 1167.77, and F = 4629.64, P 〈 0.01 ） higher than control eye by Western blotting （ 193.88 ± 8.84 vs 117.38 ± 10.76, 202.65 ± 8.37 vs 134.13± 5.06, 283.63 ±5.65 vs 186. 88 ± 7. 14, and 284.75 ±5.50 vs 183.0 ±5.58）, while by RT-PCR （200.50 ±3.25 vs 181.88 ±9.36,200.13 ± 2.95 vs 181.75±5.85, 201.88±3. 14 vs 179.25 ±9.21, and 195.50 ±3.55 vs 179.75 ±7. 12）, respectively. Conclusion The increased expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in lamina cribrosa in rat OHT eye indieates that MMPs and TIMPs may be involved in the pathogenesi

关 键 词：青光眼 巩膜 明胶酶类 金属蛋白酶类组织抑制剂 

分 类 号：R686[医药卫生—骨科学]