bFGF转染骨髓基质细胞培养及修复软骨缺损的动物试验  

Biocompatibility of polylactic-co-glycolic acid for culturing bFGF gene-transfected bone marrow stromal cells and application of the cell complex for repairing rabbit cartilage defect

作　　者：徐忠世[1] 肖德明[2] 林博文[1] 卢小虎[1] 李冉[1] 

机构地区：[1]深圳市人民医院骨科,暨南大学第二临床医学院,广东深圳518020 [2]深圳市第二人民医院,广东深圳528010

出　　处：《南方医科大学学报》2009年第6期1123-1126,共4页Journal of Southern Medical University

基　　金：深圳市科技计划项目(JH200505260325A)

摘　　要：目的探讨bFGF基因转染骨髓基质干细胞后在PLGA培养生长的生物相容性,以及采用组织工程方法培养的骨髓基质细胞-PLGA复合物修复软骨缺损动物模型的可行性。方法将bFGF基因转染成功后的骨髓基质细胞在PLGA培养生长,制备软骨缺损动物模型,将复合物植入软骨缺损,通过大体标本观察、组织学观察及Ⅱ型胶原免疫组化及RT-PCR检测对软骨缺损模型的修复效果。结果bFGF基因转染骨髓基质干细胞后可以在PLGA上正常生长,并有Ⅱ型胶原的高表达,将复合物植入动物后,可以修复软骨缺损动物模型。结论PLGA与转染后的骨髓基质细胞具有良好的生物相容性,转染后的骨髓基质细胞与PLGA复合物可以修复兔软骨缺损动物模型,可以作为自体软骨移植的一种替代治疗方法。Objective To evaluate the biocompatibility of polylactic-co-glycolic acid （PLGA） for culturing bFGF gene-transfected bone marrow stromal cells （BMSCs） and assess the feasibility of this cell complex for repairing cartilage defect in rabbits using tissue engineering method. Methods BMSCs transfected by bFGF gene were cultured on PLGA matrix to assess the biocompatibility of PLGA. The cell complex was then implanted into the cartilage defect in rabbits, and its effect in cartilage defect repair was evaluated by histological observation and immunohistochemical staining. Results BMSCs transfected by bFGF gene grew normally on PLGA matrix. After implantation, the complex showed good effect for cartilage defect repair in rabbits. Conclusion PLGA has good biocompatibility with the transfected BMSCs, and the cell complex can be used for repairing rabbit cartilage defect and may potentially serve as a substitute of cartilage autograft.

关 键 词：骨髓基质细胞 组织工程 软骨缺损 碱性成纤维细胞生长凶子 聚乳酸-羟基乙酸共聚物 

分 类 号：R684[医药卫生—骨科学]