黄芪注射液对TNF-α诱导人脐静脉内皮细胞炎症因子的影响及其分子机制  

Effects of Astragalus Membranaceus Injection on TNF-α-induced release of inflammatory factors from HUVECs and the molecular mechanisms

作　　者：叶泽兵[1] 汪凡军[2] 张刚庆[1] 杨旺民[1] 覃海森[1] 刘兴涛[1] 

机构地区：[1]广东省第二人民医院急诊科,广东广州510317 [2]广州金域医学检验中心,广东广州510330

出　　处：《南方医科大学学报》2009年第6期1137-1140,共4页Journal of Southern Medical University

基　　金：广东省中医药局科研基金(2008377)

摘　　要：目的观察黄芪注射液对肿瘤坏死因子-α(TNF-α)诱导的体外培养人脐静脉血管内皮细胞(HUVECs)核因子-κβ(NF-κβ)激活与白介素-6(IL-6)、可溶性细胞间黏附分子-1(sICAM-1)分泌的影响,探讨其分子机制。方法体外培养的第2代HUVECs用于实验,免疫组织化学分析检测细胞NF-κβ亚单位p65的表达程度,Western blot检测核内NF-κβ含量变化,ELISA法检测培养液中IL-6、ICAM-1浓度。结果TNF-α有效激活NF-κβ并诱导HUVECs分泌IL-6、sICAM-1增加;黄芪注射液预先孵育2h能抑制NF-κβ表达并减轻TNF-α诱导的上述反应。结论TNF-α可能通过激活NF-κβ使IL-6、sICAM-1增加,从而损伤血管内皮功能;黄芪注射液对上述反应有拮抗作用,推测是通过抑制NF-κβ途径实现血管内皮保护功能。Objective To investigate the protective effect of Astragalus Membranaceus Injection on human umbilical vein endothelial cells （HUVECs） against tumor necrosis factor α （TNF-α. Methods Cultured passage 2 HUVECs were stimulated with TNF-α with or without a 2-h Astragalus Membranaceus Injection treatment. The expression of nuclear factor-κβ （NF-κβ） subunit p65 were evaluated by immuncytochemistical method, and the levels of p65 in the nuclei and the protein Iκβ in the cytoplasm were evaluated by Western blotting. The levels ofinterleukin-6 （IL-6） and soluble intracellular adhesion molecule-1 （sICAM- 1） in the cell culture were determined with ELISA. Results TNF-α induced the activation of NF-κβ and increased the expressions of IL-6 and sICAM-1 in HUVESs VECs. The activation of NF-κβ by TNF-α was suppressed by Astragalus Membranaceus Injection in a dose-dependent manner. Conclusion Astragalus Membranaceus Injection can inhibit the TNF-α-induced expression of IL-6 and sICAM-1 by suppressing NF-κβ activation, suggesting its protective effect on the endothelial function.

关 键 词：黄芪注射液 肿瘤坏死因子-α 脐静脉内皮细胞 核因子-κβ 白介素-6 细胞间黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]