靶向survivin基因RNAi沉默表达对恶性胶质瘤细胞侵袭的影响  

Effect of RNA interference targeting-survivin on the invasiveness of human glioma cells in vitro

作　　者：史德刚[1] 范钰[2] 朱夫[2] 张积仁[1] 

机构地区：[1]南方医科大学珠江医院肿瘤中心,广东广州510282 [2]江苏大学附属人民医院肿瘤研究所,江苏镇江212002

出　　处：《南方医科大学学报》2009年第6期1156-1158,1162,共4页Journal of Southern Medical University

基　　金：中国博士后科学基金(2003033547)

摘　　要：目的探讨存活素(survivin)基因对胶质瘤细胞侵袭的影响和可能机制。方法应用survivin基因小干扰RNA(siRNA)转染处理胶质瘤SNB19细胞后,分别采用荧光实时定量RT-PCR和Western blot检测survivin基因mRNA和蛋白水平,分别采用软琼脂集落培养试验和Boyden小室模型试验检测癌细胞集落生长数和侵袭力。采用Western blot方法检测癌细胞尿激酶纤溶酶原激活物(uPA)蛋白水平。结果survivin siRNA转染组细胞的survivin基因mRNA和蛋白水平明显下调,转染组胶质瘤细胞集落生长数和癌细胞穿膜细胞数明显下调,且与浓度相关。转染组癌细胞uPA蛋白水平明显下降。结论Survivin基因在胶质瘤细胞侵袭中起着重要作用,采用靶向survivin siRNA转染可抑制其侵袭能力,其机制可能与下调uPA基因表达有关。Objective To study the role of survivin gene in the invasive behavior of glioma cells and explore the possible mechanism. Methods The mRNA and protein expressions of survivin in glioma cell line SNB19 transfected by small interfering RNA （siRNA） targeting survivin were determined by real time RT-PCR and Western blotting, respectively. The anchorage-independent growth of the cells was examined by clone formation assay in soft agar, and their invasiveness was evaluated using a Boyden chamber model. The protein level of urokinase-type plasminogen activator （uPA） was also determined by western blotting. Results Survivin siRNA dose-dependently inhibited the anchorage-independent growth and invasiveness and reduced the expression ofuPA protein in SNB19 cells. Conclusion RNA interference targeting survivin can inhibit the invasiveness of glioma cells in vitro possibly by down-regulating uPA expression.

关 键 词：胶质瘤 存活素 侵袭 尿激酶纤溶酶原激活物 

分 类 号：R739.4[医药卫生—肿瘤]