双室体描仪测定豚鼠气道阻力和气道反应性  

Measurement of airway resistance and reactivity in guinea pigs using double-chamber plethysmography

作　　者：姚卫民[1] 赖克方[1] 罗远明[1] 刘春丽[1] 陈如冲[1] 罗炜[1] 钟南山[1] 

机构地区：[1]呼吸疾病国家重点实验室(广州医学院)/广州医学院第一附属医院,广东广州510120

出　　处：《南方医科大学学报》2009年第5期841-843,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30370621);广州市科技攻关重点项目(2002Z2-E0091)

摘　　要：目的建立应用双室体描仪测定豚鼠气道阻力和气道反应性的方法,为哮喘的研究提供有效手段。方法应用双室体描仪分别测定乙酰甲胆碱(Mch)激发后豚鼠气道阻力的回复时间;于实验的第1、15天分别测定正常对照组豚鼠气道阻力和气道反应性2次,每次间隔2h,验证测定结果的重复性;观察OVA致敏豚鼠OVA激发前后气道阻力和气道反应性的变化情况。结果PC100浓度的Mch雾化后,豚鼠在1h内气道阻力回到基线水平。正常对照组豚鼠在第1、15天分别测量两次的基础气道阻力sRaw为(3.25±0.67)cmH2O·s、(3.33±0.58)cmH2O·s、(3.30±0.56)cmH2O·s、(3.32±0.75)cmH2O·s,气道反应性(log2PC100)值分别为8.48±0.94、8.64±1.04、8.56±0.67、8.64±0.60,气道阻力和气道反应性两两比较,差异无统计学意义(P>0.05)。致敏豚鼠OVA激发后气道阻力和气道反应性均较激发前显著提高,sRaw分别为(7.08±1.82)cmH2O·s和(2.87±0.53)cmH2O·s(P<0.01),log2PC100值分别为6.64±1.26和8.48±1.17(P<0.01)。结论成功建立了应用双室体积描记法测定豚鼠气道阻力和气道反应性的实验方法。Objective To establish a method for measurement of airway resistance （sRaw） and reactivity in guinea pigs. Methods Methacholine spray at gradient concentrations was given to guinea pigs. PC100 was defined as the concentration of methacholine when the sRaw doubled in the guinea pigs using a double-chamber plethysmograph. The time for the recovery of PC100 resistance to baseline levels was measured. The sRaw and PC100 were measured twice on days 1 and 15 （4 time points） in the guinea pigs before and after OVA challenge. Results PC100 in a normal guinea pig airway was shown to recover the baseline level within 1 h. Double-chamber plethysmographical measurement ＆the sRaw and PC100 in normal guinea pigs did not show significant differences between the time points [sRaw： 3.25±0.67, 3.33±0.58, 3.30±0.56, and 3.32±0.75 cmH20·s; log2PC100： 8.48±0.94, 8.64±1.04, 8.56±0.67, and 8.64±0.60, respectively, P〉0.05]. The sRaw and airway reactivity were significantly increased in guinea pigs challenged with OVA [sRaw： 7.08±1.82 vs 2.87±0.53 cmH20·s, P〈0.01; log2PC100： 6.64± 1.26 vs 8.48 ± 1.17, P〈0.01 ]. Conclusion A double-chamber plethysmography for measurement of sRaw and airway reactivity in guinea pig is established successfully.

关 键 词：体描仪 气道阻力 气道反应性 豚鼠 

分 类 号：R562.25[医药卫生—呼吸系统]