特布他林短时间作用增强急性肺损伤大鼠肺泡液清除的机制与αENaC的相关性  

Role of epithelial sodium channel α subunit in terbutaline-induced transient enhancement of pulmonary edema clearance in adult rats with acute lung injury

作　　者：黄冬祥[1] 李涛平[1] 何琳[1] 

机构地区：[1]南方医科大学南方医院呼吸科,广东广州510515

出　　处：《南方医科大学学报》2009年第5期868-871,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30471720);广东省自然科学基金(04020458)

摘　　要：目的探讨β2受体激动剂特布他林短时作用增强成体大鼠肺泡液清除的机制与肺泡上皮钠离子通道α亚基(αENaC)的相关性。方法建立油酸急性肺损伤(ALI)大鼠模型;以PaO2、氧合指数,肺组织HE染色,血管外肺水含量(EVLW)为指标验证特布他林经气管给药作用1h促进ALI大鼠肺泡液重吸收的效果;通过实时荧光定量RT-PCR、Western blot测定各组大鼠肺组织的αENaC mRNA、αENaC蛋白的表达并比较它们的差异。结果特布他林治疗组大鼠的PaO2、氧合指数、EVLW较ALI组有显著差异;各组大鼠αENaC mRNA、αENaC蛋白表达差异无统计学意义。结论特布他林经气管给药作用1h能显著促进ALI大鼠肺泡液重吸收;特布他林短时作用增强成体大鼠肺泡液清除没有通过αENaC基因表达实现。Objective To explore the association of epithelial sodium channel α subunit （αENaC） with terbutaline-induced transient enhancement of pulmonary edema clearance in adult rats with acute lung injury （ALl）. Methods The effect of 1-h intratracheal terbutaline treatment on pulmonary edema clearance in adult rats with experimental ALI was observed by blood gas analysis, lung tissue HE staining, and extravascular lung water （EVLW） content measurement. The mRNA and protein expressions of αENaC in the lung tissues were detected by fluorescence quantitative real-time RT-PCR and Western blotting, respectively. Result Terbutaline treatment of the rats with ALI resulted in significant differences in PaO2, oxygenation index, and EVLW from those in ALI group without treatment. No significant differences in pulmonary αENaC mRNA and protein expressions were noted between the normal control, ALI, and terbutaline-treated ALI groups. Conclusions Intratracheal terbutaline administration for 1 h can significantly promote pulmonary edema clearance in adult rats with ALI, and this effect is not mediated by aENaC gene expression.

关 键 词：急性肺损伤 上皮钠离子通道 特布他林 血管外肺水 

分 类 号：R96[医药卫生—药理学]