雌激素对化疗损伤性卵巢早衰保护作用的实验研究  

Protective effect of estrogen against chemotherapy-induced ovarian damage in rats

作　　者：韩丽萍[1] 何援利[1] 

机构地区：[1]南方医科大学珠江医院妇产科,广东广州510282

出　　处：《南方医科大学学报》2009年第5期872-875,共4页Journal of Southern Medical University

基　　金：广东省科技计划项目(2007B030501005)

摘　　要：目的观察雌激素对环磷酰胺(CTX)所致大鼠卵巢功能损伤的保护作用。方法选用具有正常性周期的2～3月龄雌性Wister大鼠60只,随机分成正常对照组、CTX组、E2组、CTX+E2组,分别给予生理盐水、CTX腹腔注射、戊酸雌二醇灌胃和CTX与戊酸雌二醇联合给药。用药后观察并比较各组大鼠一般状态、体质量、卵巢和子宫湿重。采用放免法测定不同时期各组大鼠血清中卵泡刺激素(FSH)、雌二醇(E2)水平。卵巢组织病理切片HE染色观察卵巢组织结构的变化。结果CTX组大鼠与CTX+E2组比较,血清FSH浓度明显升高,E2水平明显降低(P<0.05)。CTX组大鼠卵巢和子宫湿重明显低于CTX+E2组(P<0.05)。光镜下CTX组大鼠卵巢卵泡总数较CTX+E2组显著减少(P<0.05);CTX组大鼠卵巢组织纤维化、坏死程度也较CTX+E2组明显。结论雌激素能改善CTX对卵巢功能的损伤,对CTX所致卵巢功能损伤有一定的保护作用。Objective To investigate the protective effect of estrogen against cyclophosphamide （CTX）-induced ovarian failure in female rats. Methods Sixty female Wistar rats （2-3 months old） were randomized into 4 groups to receive treatments with normal saline, CTX, estradiol （E2） or CTX＋E2. Serum estradiol and follicle-stimulating hormone （FSH） concentrations were measured regularly in the 4 groups, and the weight of the ovary and uterus, follicle number and mean diameter of the follicles were compared. Results Compared with CTX＋E2 group, the CTX group showed significantly increased FSH levels and decreased E2 levels （P〈0.05）. The weight of the ovary and uterus and follicle number were significantly lower in CTX group than in CTX＋E2 group （P〈0.05）. No obvious differences in the indexes were found between the control and CTX＋E2 groups. Conclusion Estrogen administration provides protection against CTX-induced ovarian damage in rats.

关 键 词：雌激素 卵巢早衰 环磷酰胺 卵泡刺激素 雌二醇 

分 类 号：R711.75[医药卫生—妇产科学]