液体栓塞材料2-聚甲基丙烯酸羟乙酯的研制  

Effect of renal artery embolization using 2-poly-hydroxyethyl-methacrylate as a liquid embolic agent:a study in rabbits

作　　者：杜浩[1] 马廉亭[1] 殷斌烈[2] 吴佐泉[1] 秦尚振[1] 徐国政[1] 郭再玉[1] 张新元[1] 

机构地区：[1]广州军区武汉总医院神经外科,湖北武汉430070 [2]武汉工程大学制药系,湖北武汉430073

出　　处：《南方医科大学学报》2009年第5期894-897,共4页Journal of Southern Medical University

基　　金：全军十一五攻关面上项目B类(06MB164)

摘　　要：目的研究新型液体栓塞材料2-聚甲基丙烯酸羟乙酯(2-P-HEMA)的栓塞性能及技术可行性。方法分别测定2%、3.5%、5%、6.5%、8%、9.5%2-P-HEMA的乙醇溶液(A组)、乙醇/碘比醇溶液(B组)及乙醇/三氧化二铋(Bi2O3)溶液(C组)在流动盐水中的凝固时间。分别采用2%、5%、8%2-P-HEMA的乙醇/Bi2O3溶液栓塞兔肾动脉,观察栓塞效果,术后分期复查肾动脉造影,取肾脏标本行大体及光镜检查。结果2-P-HEMA的乙醇溶液注入流动水中很快呈絮状固化。A、B、C组相互之间的各浓度亚组材料凝固时间无显著性差异(P>0.05)。中低浓度栓塞材料能顺利通过微导管,注射阻力小,能完全栓塞肾动脉及其分支,在透视下显影良好,无粘管现象。高浓度材料通过微导管阻力大,推注困难。造影复查未见肾动脉再通。肾脏标本可见肾脏动脉腔内有栓塞材料填充,自肾动脉至肾小球动脉内均可见到栓塞材料。结论2-P-HEMA能在流动水中快速凝固,合适浓度可满意栓塞兔肾动脉,是一种较为理想的非粘附性液体栓塞材料。Objective To assess the effect of a liquid embolic agent 2-poly-hydroxyethyl -methacrylate （2-P-HEMA） for renal artery embolization in rabbits. Methods The precipitation time of different concentrations （2%, 3.5%, 5%, 6.5%, 8% and 9.5%） of 2-P-HEMA dissolved in different solutions （ethanol, ethanol/iobitridol, and ethanol/Bi203） were determined in flowing water. The mixtures of 2-P-HEMA （2%, 5%, and 8%） with ethanol/Bi203 were injected into the renal arteries of the rabbits, and the artery-embolizing effects were assessed using angiography at 2 and 12 weeks after the injection, with also macroscopic and microscopic examination of the embolized kidneys. Results The mixtures of 2-P-HEMA and ethanol formed flocculent precipitation a few seconds after injection into flowing water, and the precipitation time showed no significant variations with the concentration of 2-P-HEMA in the mixture. Low and moderate concentrations of 2-P-HEMA could pass through the mierocatheter smoothly with little injection resistance, and resulted in complete occlusion of the renal arteries without adhesion to the microcatheter. Angiography at 2 and 12 weeks detected no recanalization of the occluded renal arteries. MacroscopicaUy, the lumen of the renal arteries was found to be occluded by the embolic agents, and deep penetration of the embolic agents into the glomerular arteries was observed microscopically. The mixture containing high-concentration 2-P-HEMA was difficult to deliver through the microcather due to high injection resistance. Conclusion 2-P-HEMA can be rapidly precipitated after injection into flowing water, and allows complete embolization of the renal arteries of rabbits at proper concentrations, suggesting its great potential as an endovascular liquid embolic agent.

关 键 词：2-聚甲基丙烯酸羟乙酯 栓塞 动物实验 

分 类 号：R318.08[医药卫生—生物医学工程]