不同治则中药对胆囊胆固醇结石小鼠模型的成石影响  

Effects of Chinese Herbs with Different Therapeutic Principles on Biochemistry in Model Mouse with Cholesterol Gallstone

作　　者：张静喆[1] 梁晓强[2] 顾宏刚[1] 章学林[1] 朱培庭[1] 

机构地区：[1]上海中医药大学附属龙华医院外科,上海200032 [2]上海中医药大学中医外科研究所

出　　处：《中国中西医结合外科杂志》2009年第3期291-294,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：上海市科委基础重点项目(03JC14066);上海市医学领军人才资助课题(LJ06048);上海市重点学科资助课题(T0304)

摘　　要：目的:观察中药清胆胶囊和养肝利胆颗粒对C57小鼠胆囊胆固醇结石模型成石的影响和差异。方法:应用C57BL/6J雌性10周龄小鼠建立胆固醇结石模型(n=15),并设立正常对照组(n=10)、清胆胶囊组(n=13)及养肝利胆颗粒组(n=13),观察各组动物的成石率、血清胆固醇、钙离子及肝脏丙二醛(MDA)及过氧化物歧化酶(SOD)等生化指标的差异。结果:各组动物成石率分别73.33%、0%、23.08%及30.77%(P<0.001)。模型组各项指标均出现明显异常,而清胆胶囊和养肝利胆颗粒组的各项指标均有不同程度的改善,其中二者在改善血清总胆固醇(TC)及低密度脂蛋白胆固醇(LDL-C)有明显差异(均P<0.01)。两种中药相比,清胆胶囊更能显著升高高密度脂蛋白(HDL-C)含量(P<0.05),而养肝利胆颗粒更能增强肝脏SOD活性(P<0.05)。4组间血清Ca2+浓度无显著性差异。结论:疏肝清热利胆中药清胆胶囊和养肝柔肝中药养肝利胆颗粒均可降低小鼠胆固醇结石模型成石率,改善与成石相关的血清及肝脏等生化学异常,二者均呈多靶点作用,但仍存在着一定的差异。养肝利胆颗粒在增强肝脏SOD活性方面优于清胆胶囊。Objective To observe the effects of Chinese herbal medicines QingDan （清胆） capsule （QDJN） and YangGanLiDan（养肝利胆） granule （YGLD）, both with different therapeutic principles, on biochemistry in C57 mouse model with cholesterol gallstone. Methods Animal model of cholesterol gallstone was reproduced in C57BL/6J mouse by feeding high cholesterol diet. And the C57BL/6J mice were randomly divided into normal control group （n = 10）, model group （n = 15）, QDJN group （n = 13） and YGLD group （n = 13）. After 6 - week different feeding experience animals were sacrificed and sampled to calculate the rate of stone formation, and the serum cholesterol and Ca2 ＋ and the hepatic malonaldehyde （MDA） and superoxide dismutase （SOD） levels determined. Results The incidence of stone formation in four groups was 11/15 in model group, 0/10 in normal control group, 3/13 in QDJN group and 4/13 in YGLD group （ P 〈 0. 001 ）. All the serum biochemical indices were abnormal significantly in model group, compared with QDJN and YGLD groups. And the difference of TC and LDL - C between QDJN and YGLD groups weren＇ t obvious. The SOD in YGLD group was higher than in QDJN group （ P 〈 0.05）. There wasn＇ t a significant difference of Ca2 ＋ density among the four groups. Conclusion Both Chinese herbal medicines with different therapeutical principle （QD- JN to disperse the depressed liver- energy and promote bile secretion, YGLD to nourish and modulate liver- yin） can reduce the rate of stone formation, improve biochemical abnormality in muhilevels. But there was some disparity between QDJN and YGLD groups. QDJN had a better effect on HDL - C, and YGLD had a better effect on the activities of SOD.

关 键 词：养肝利胆颗粒 清胆胶囊 胆固醇结石 小鼠 

分 类 号：R657.42[医药卫生—外科学] R285.6[医药卫生—临床医学]