大鼠全脑照射后早期血清S-100B变化特征  

Alteration of S-100B in serum following whole-brain irradiation in rats

作　　者：毛卫东[1] 田野[1] 

机构地区：[1]苏州大学附属第二医院放疗科,江苏苏州215004

出　　处：《中华放射医学与防护杂志》2009年第3期271-273,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：江苏省卫生厅重大科研课题资助项目（K200406）;苏州大学医学发展基金资助项目（EE123504）.

摘　　要：目的观察大鼠全脑不同剂量照射后1月内不同时间点血清中S-100B的变化，以探讨S-100B在早期放射性脑损伤中的变化特征。方法对大鼠予4MeV电子线分别单次全脑照射2、10和30Gy制备大鼠早期放射性脑损伤模型。采用酶联免疫吸附试验（ELISA）检测照射后1h、6h、12h、24h、3d、1周、1个月血清中S-100B的浓度。结果大鼠血清S-100B浓度在照射后6h升高最明显，照射后12h浓度下降，照后24h再升高（除外2Gy组为在照射后3d再升高），随后下降直到最后观察时间点。结论大鼠全脑照射后血清S-100B水平发生了变化，不同剂量组血清S-100B浓度有显著性差异，剂量越高，浓度越高。结果提示S-100B可能会成为检测早期放射性脑损伤的重要血清指标。Objective To investigate the change of S-100B in serum in rats at various times after exposure to different whole-brain irradiation doses and to explore the characteristics of the S-100B change in early radiation-induced brain injuries. Methods A model of brain radiation-induced injuries was established in the rat after whole-brain irradiation with a single dose of 2, 10, or 30 Gy of 4 MeV electron. The concentration of S- 100B in serum was detected by means of enzyme-linked immunosorbent assay （ELISA） at different time points （1,6,12,24,3 days,1 week,1 month） after exposure. Results In terms of measuring time points, the serum concentration in all groups reached the first peak 6 hours after irradiation, declined after 12 hours, rose again after 24 hours, and started to decline again until the last time point （1 month）, with the exception of the 2 Gy group, which reached the second peak at 3 days after irradiation. Conclusions The S-100B serum concentration was changed in the rats after whole-brain irradiation and these changes are positively related to the irradiation amount. It seems that the higher the dose, the higher the serum concentration and the differences between the dose groups are significant （via multiple regression models） . The S-100B may be a valuable serum marker for the brain irradiation injury.

关 键 词：放射性脑损伤 血清 S-100B 酶联免疫吸附试验(ELISA) 

分 类 号：R818[医药卫生—放射医学]