血管内皮生长因子、转化生长因子β1、p53在晚期日本血吸虫病肝组织中的表达及意义  

Expression and significance of VEGF,TGF-β1 and p53 in the liver tissue from advanced schistosomiasis Japonica patients

作　　者：陈栋良[1] 郭继雄 刘保安[3] 邓维成 李静[1] 赵晓贡 

机构地区：[1]湖南省血吸虫病防治所附属湘岳医院病理科,岳阳市414000 [2]湘岳医院外科 [3]中南大学湘雅医学院病理学系

出　　处：《中华实用诊断与治疗杂志》2009年第6期528-530,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：湖南省卫生厅重点课题资助项目(A2004-2006);湖南省卫生厅科研基金课题资助项目(C2004-034)

摘　　要：目的:研究血管内皮细胞生长因子、转化生长因子β1和p53蛋白在晚期血吸虫病患者肝脏组织中的表达及其临床意义。方法:采用免疫组织化学法链霉菌抗生物素蛋白-过氧化物酶连结法检测95例晚期血吸虫病、20例原发性肝细胞癌、10例正常肝组织中血管内皮细胞生长因子、转化生长因子β1和p53的表达情况,并与临床病理指标比较分析。结果:95例晚期血吸虫病肝组织中转化生长因子β1、血管内皮细胞生长因子、p53蛋白阳性表达分别为74.74%、64.21%、8.42%,转化生长因子β1、血管内皮细胞生长因子阳性表达的强度与肝炎分级、肝纤维化分期或合并乙肝病毒感染呈正相关(P均<0.05),转化生长因子β1与血管内皮细胞生长因子阳性表达呈正相关(P<0.01);p53在晚期血吸虫病中的阳性表达明显低于原发性肝细胞癌(P<0.01);正常肝组织中未观察到转化生长因子β1、血管内皮细胞生长因子和p53蛋白表达。结论:血管内皮细胞生长因子、转化生长因子β1与肝纤维化的发生、发展密切相关,二者可作为晚期血吸虫病患者肝纤维化的诊断和疗效评估指标;血管内皮细胞生长因子在晚期血吸虫病中高表达,提示抗血管生成可能为肝纤维化和门静脉高压症防治提供新的思路,p53蛋白在晚期血吸虫病中有低表达,对探讨血吸虫感染与原发性肝细胞癌的关系有一定意义。Objective To study the clinical significance of the expression of VEGF/TGF-β1/p53 in liver tissues from advanced schistosomiasis patients. Methods The expression of VEGF/TGF-β1/p53 protein was observed in the liver tissues from 95 cases of advanced schistosomiasis, 20 cases of hepatocellular carcinoma and 10 cases of normal samples with immunohistochemical SP. The results were compared with the clinicopathological parameters. Results In 95 cases of the advanced schistosomiasis, the positive expression rates of TGF-β1, VEGF, p53 protein were 74. 74%, 64.21%,8.42% respectively. The expressions of TGF-β1 and VEGF were correlated with hepatitis stage, liver fibrosis stage, and whether the merger of hepatitis B virus infection（all P〈0.05）, and the expression of TGF-β1 protein was correlated with VEGF （P 〈 0. 01）. In the livers of schistosomiasis patients, the expression of p53 was significantly lower than hepatocellular carcinoma （P〈0.01）, and the expressions of TGF-β1, VEGF and p53 protein were not checked in normal liver tissues. Conclusion The expression of VEGF and TGF-β1 are closely related with the occurrence and development of liver fibrosis, and both of them can be used as diagnotic parameters and evaluation of therapeutic effect in liver fibrosis schistosomiasis patients. The high expression of VEGF in the schistosomiasis patients discloses that anti-angiogenesis may be a new way in diagnosis and treatment of liver fibrosis and portal hypertension. However the low expression of p53 in schistosomiasis patients suggests that the schistosomiasis patients is correlated with tumorigenesis.

关 键 词：晚期血吸虫病 血管内皮生长因子 转化生长因子Β1 P53 免疫组织化学 

分 类 号：R532.21[医药卫生—内科学]