铜元素诱导烟草抗马铃薯Y病毒脉坏死株系机理研究  

A Study on the Mechanism of Copper-Induced Resistance to Potato VirusY-Vein Necrosis Strain (PVY^N) in Tobacco

作　　者：李鑫[1] 顾晶晶[1] 赵秀香[1] 李立梅[1] 吴元华[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161

出　　处：《病毒学报》2009年第3期226-230,共5页Chinese Journal of Virology

基　　金：辽宁省科技攻关项目(2006215004)资助

摘　　要：采用病情指数调查、DAS-ELISA和生理生化指标测定的方法,研究了铜(Cu)元素对烟草抗马铃薯Y病毒脉坏死株系(Potatovirus Y-vein necrosis strain,PVYN)的室内效果及对植株抗病性的诱导作用。结果表明:喷施不同浓度的铜元素均能延迟烟株显症和叶脉出现褐色坏死时间,降低发病程度,烟株体内病毒含量也明显减少,当Cu元素浓度为0.8mg/L时,效果最显著。单独喷施Cu元素(0.8mg/L)和喷施Cu元素后再接种PVYN的处理,均能提高烟株体内叶绿素a、叶绿素b含量及苯丙氨酸解氨酶活性;另外,单独喷施Cu元素处理烟株后能诱导总酚和类黄酮含量的提高,但喷施Cu元素后接种PVYN的烟株中总酚和类黄酮的含量在接种处理后3d内迅速上升高于对照,随后下降,在6d后虽又呈缓慢上升趋势,但仍明显低于对照。总酚和类黄酮的含量与叶脉褐变密切相关,因此总酚和类黄酮含量的降低可减轻植株褐变。研究表明,Cu元素具有诱导抗病性、增强烟草对PVYN侵染的抵抗能力。In order to reveal the induced resistantce mechanism of tobacco treated with copper solution to potato virus Y-vein necrosis strain （PVY^N）, disease indexes, contents of virus and some physiological and biochemical indexes in tobacco were studied. The results showed that when treated at the copper concen- tration of 0. 8 mg·L^-1 , the symptom displayed and vein necrosis on tobacco were postponed, the disease index and content of virus sharply decreased , and the content of chlorophyll a, chlorophyll b and phenylalanine ammonia lyase （PAL） activity remarkably increased. Furthermore, vein necrosis closely linked to contents of total phenol and flavonoid. In this study, the contents of total phenol and flavonoid were promoted when treated with a solution at the copper concentration of 0.8 mg·L^-1. But the contents of total phenol and flavonoid reached to the first peak at the 3rd day after inoculation, and then decreased to the lowest levels which even were lower than those of the control after inoculating PVY^N. Then the contents of total phenol and flavonoid increased slowly from the 6td but still lower than those of the control. The result implied that spraying copper solution might play an important role in induced resistance of tobacco to vein necrosis disease and strengthen the antiviral capability to PVY^N.

关 键 词：烟草 铜 马铃薯Y病毒脉坏死株系 诱导抗性 

分 类 号：S432.41[农业科学—植物病理学]