介入法犬股骨头坏死模型制作  

Preparation of femoral head necrosis model in canine with endovascular technique

作　　者：马坤[1] 顾建平[1] 苏浩波[1] 楼文胜[1] 何旭[1] 陈亮[1] 陈国平[1] 毛存南[1] 宋进华[1] 汪涛[1] 

机构地区：[1]南京医科大学附属南京第一医院介入科,210006

出　　处：《介入放射学杂志》2009年第6期470-473,共4页Journal of Interventional Radiology

摘　　要：目的应用介入方法建立犬股骨头坏死模型。方法9只成年杂种犬全麻后,分别通过股动脉Seldinger法穿刺插管,超选一侧股骨头供血动脉行平阳霉素灌注及明胶海绵栓塞处理,建立股骨头缺血坏死模型,对侧股骨头作对照。模型犬随机分为3组,每组3只,分别于栓塞术后2、4、6周处死。所有犬均于栓塞术前及术后每周行双侧髋关节MRI扫描,栓塞术前、术后即刻、处死前行双侧股骨头供血动脉DSA造影检查,最后一次造影结束处死动物,取双侧股骨头标本送病理学观察。结果9只犬均造模成功,术后所有栓塞动脉造影复查均未见再通。造模侧股骨头在术后2周出现早期病理学改变,术后4周MRI显示股骨头坏死异常信号,病理切片出现不同程度骨细胞及骨小梁坏死,术后6周病理切片可见坏死后增生及修复反应。结论本法能成功制作出犬股骨头坏死模型,具有创伤小、造模方法简单、成模时间短、动物死亡率低等优点,是一种比较理想的造模方式。Objective To establish a femoral head necrosis model in canine by using endovascular technique. Methods After general anesthesia, catheterization with Seldinger technique via femoral access was carried out in 9 healthy hybrid adult dogs. When super-selective catheterization of the feeding artery of one-side femoral head was accomplished, Pingyangmycin and gelfoam particles were injected through the catheter to embolize the artery, and the femoral head necrosis model was thus established, with the offside femoral head being served as control. The model dogs were randomly and equally divided into three groups. The dogs in group A, B and C were sacrificed in 2, 4 and 6 weeks after the procedure respectively. MRI scanning of both coax joints was performed in all dogs before the procedure and once a week after the procedure, while DSA examination of the feeding arteries of both femoral beads was carried out before and immediately after the embolization, as well as immediately before sacrifice. The femoral heads were taken out and sent for pathologic examination. Results The femoral head necrosis model was successfully established in all 9 dogs, and re-open of the embolized arteries was not found on follow-up DSA. Early pathological changes appeared 2 weeks after the operation. Pathologically, a greater or less degree of osteocyte and bone trabecnla necrosis was observed 4 weeks after the procedure, which was manifested as abnormal signal indicating femoral head necrosis on MRI scan. Proliferation and restoration changes were seen on pathological section 6 weeks after the operation. Conclusion Canine model of femoral head necrosis can be prepared with endovascular technique. This technique is minimally-invasive, technically-simple and quickly-prepared with low mortality, therefore, it is an ideal preparation way.

关 键 词：股骨头坏死 动物模型 血管内介入 犬 

分 类 号：R681.8[医药卫生—骨科学]