双水相体系中C_(21)甾苷C_(11) α-羟化研究  

C_(11) α-Hydroxylation of C_(21) Steroidal Aglycones in Aqueous Two-Phase

作　　者：李于善[1] 贺艳[1] 

机构地区：[1]三峡大学天然产物研究与应用湖北省重点实验室,宜昌443002

出　　处：《药物生物技术》2009年第3期245-249,共5页Pharmaceutical Biotechnology

基　　金：湖北省教育厅项目;基金编号:2004D003

摘　　要：对白首乌C21甾苷元告达庭甾苷元和开德甾苷元（Ⅱ）实现C11α-羟基化，要求副产物少，收率高。采用赭曲霉（Aspergillus ochraceus）和犁头霉（Absidia sp．）两种微生物在双水相体系中同步转化的方法，羟化反应中控制pH值在6．0～5．0，于30℃温度下振荡培养70h；结果，产物经IR、MS、NMR分析确认是C11α-羟基化了的告达庭甾苷元（Ⅲ）和开德甾苷元（Ⅲ），副产物少，收率达70．1％。赭曲霉（Aspergillus ochraceus）和犁头霉（Absidia sp．）两种微生物在双水相体系中同步转化的方法是实现C11α-羟基化的有效方法。Two types of Cynanchum auriculatum C21 steroidal aglycones （ Ⅱ ）, such as Caudatin steroidal aglycones and Kaider steroidal aglycones, were C11α-hydroxylated, with a little byproduct and high productivity. C21 steroidal aglycones, namely, Caudatin steroidal aglycones and Kaider steroidal aglycones （Ⅲ） were prepared by biotransformation with Aspergillus ochraceus and Absidia sp. In aqueous two- phase system at pH 6.0-5.0, 30℃ and 70 h. The results showed that the products were identified as two of C11α- hydroxylated cynanchum auriculatum Caudatin steroidal aglycones and Kaider steroidal aglycones（Ⅲ） by IR, MS, and NMR. Two microorganisms （Aspergillus ochraceus and Absidia sp. ） enhanced the C11α- hydroxylation reaction of C21 steroidal aglycones simulteously in aqueous two-phase system effeetively.

关 键 词：赭曲霉 犁头霉 C11α-羟基化 同步转化 双水相系统 

分 类 号：R284[医药卫生—中药学]