丹参杂交育种及其异源四倍体的诱导与鉴定  

Allotetraploid Induction and Identification of Salvia Miltiorrhiza Bunge and Its Hybridization Breeding

作　　者：刘竟飞[1] 高山林[1] 黄和平[1] 刘利[1] 李晓瑜[1] 

机构地区：[1]中国药科大学,南京210038

出　　处：《药物生物技术》2009年第3期260-264,共5页Pharmaceutical Biotechnology

摘　　要：通过杂交育种技术对丹参的两个不同品种进行杂交，得到的子一代试管苗经同工酶电泳分析，根据亲本与子代之间农艺性状的差异，鉴定得h-1等几个杂交种株系。并进行了杂交株系田间农艺性状观察比较及根部药材主要有效化学成分的HPLC测定。对杂交种F1代大量扩繁后，采用秋水仙碱进行化学诱导异源多倍体，并鉴定所得诱导株系。试验结果表明秋水仙素浓度为（10～15）μg／g是诱导多倍体的最适宜浓度，并鉴定得到hz4-3、hz4-11等7个异源四倍体株系。为进一步选育异源多倍体优良品种奠定了基础。Two lines Salvia Miltiorrhiza Bunges which came from different provinces of China were hybridized by the hybridization breeding technique. The first generation was detected by peroxidase isozyme electrophoresis and the difference agronomic character between the line parents and the first generation was analyzed. As a result, besides h-7, there are several lines which are real Hybridizations.Hybridization lines were selected according to agronomic characteristics and roots yields in fields of experimental plots. The dried roots of effective lines were used for the determination of effective chemical components by HPLC to evaluate the quality of crude drug. The first generation is treated which has been breeded a great deal with colchicine to induce allotetraploid plant and then its induced lines were detected. The results indicated that the best content of colchicine to induce polyploidy plant is 10-15μg/ g. and attained 7 allotetraploids which contain hz4-3, hz4-11. It laid the foundation for developing an excellent allopolyploidy plant.

关 键 词：丹参 杂交 同工酶电泳 异源四倍体 多倍体 

分 类 号：R284.1[医药卫生—中药学] R384.1[医药卫生—中医学]