免疫鸡群肾型传染性支气管炎病毒的分离和鉴定  

Separation and Identification of Kidney-type Infectious Bronchitis Virus from Immunized Chicken

作　　者：徐雪[1] 孙彦婷[1] 王宏魁[1] 王泽霖[1] 常洪涛[1] 杨霞[1] 陈陆[1] 王川庆[1] 

机构地区：[1]河南农业大学禽病研究所,河南郑州450002

出　　处：《安徽农业科学》2009年第19期8881-8882,8887,共3页Journal of Anhui Agricultural Sciences

基　　金：中德合作项目资助

摘　　要：[目的]鉴定免疫鸡群肾型传染性支气管炎病毒（IBV）。[方法]以接种IBV的鸡胚尿囊液为模板，根据GenBank中登录的IBV的N基因序列设计引物，通过RT-PCR扩增出目的片段，经酶切鉴定后对其进行测序鉴定。[结果]PCR扩增片段长度为409bp，将其Ⅳ基因核苷酸序列与不同地区分离株（山东、黑龙江等）以及欧洲呼吸型疫苗株（M41）的IBV核苷酸序列进行比较，结果表明，不同毒株的核苷酸同源性在85．6％～100．0％，此次分离所得IBV的N基因与IBVSD0708株（EU352625）N基因的核苷酸同源性高达99．3％，而与呼吸型疫苗株M41（M28566）的同源性仅为87．3％。测序结果及序列分析可以证实分离到的病毒为IBV，命名为HN／HL株。[结论]该研究为鸡传染性支气管炎病毒的控制奠定了基础。[ Objective] The aim was to identify kidney-type infectious bronchitis virus （IBV） from immunized chicken. [ Method ] The target fragment was amplified from the allantoic fluid mRNA of IBV by RT-PCR using the primers designed from N gene sequence of IBV entered in GenBank and after identified with enzyme restriction, it was sequenced. [ Result] The, length of the amplified fragment was 409bp. Compared its N gene nucleotide acid sequence with IBV gene nuclentide acid sequence from isolated strains in different areas such as Shandong and Hei- longjiang and European respiratory vaccine strain M41, the result showed that the nucleotide acid homology among different strains was between 85.6% and 100.0%. The N gene nucleotide acid homology between the separated IBV and IBV SD0708 strain was up to 99.3%, and that between the separated IBV and respiratory vaccine strain M41 was only 87.3%. The sequencing result and sequence analysis could confirm the isolated virus was IBV, which was named HN/HL strain. [ Conclusion] The research laid the foundation for the control of chicken infectious bronchitis virus.

关 键 词：免疫鸡群 传染性支气管炎病毒 鉴定 N基因序列分析 

分 类 号：S852.65[农业科学—基础兽医学]