丽江塔城地区云南红豆杉内生真菌的多样性研究  

Diversity of Endophytic Fungi from Taxus yunnanensis Cheng et L.K.Fu of Tacheng Township in Lijiang City

作　　者：郑法新[1] 程璐[1] 李侠[1] 邵士成[2] 刘群[1] 

机构地区：[1]日照职业技术学院,山东日照276826 [2]中国科学院昆明植物研究所,云南昆明650204

出　　处：《安徽农业科学》2009年第19期8885-8887,共3页Journal of Anhui Agricultural Sciences

基　　金：山东省中青年科学家科研奖励基金(2007BS02010)

摘　　要：为了探悉云南红豆杉内生真菌的多样性,研究了云南丽江维西地区云南红豆杉(Taxus yunnanensisCheng et L.K.Fu)内生真菌的种类组成及数量。从云南红豆杉根、茎、叶中分离获得115种内生真菌,经过形态学和ITS分析结果表明,这115种内生真菌分属于21属,其中分布最广的类群是芽枝霉属(19种),其次是黑孢属(17种)和拟茎点霉属(14种),说明云南红豆杉不同部位内生真菌的数量、分布、种群及其组成存在差异,具有丰富的物种多样性。经初步检测,其中有4个菌种能够产生紫杉醇。In order to explore the endophytic fungi diversity in Taxus yunnanensis Cheng et L. K. Fu, the composition and quantity of endophytic fungi was studied. 115 endophytes were isolated and obtained from yew tree, Taxus yunnanens/s. According to the morphological characterization and ITS gene sequences, these 115 species belonged to 21 genera. Among these, Cladosporium sp. was the most prevalent genus, followed by Nigrospora sp. and Phomopsis . The results showed that the quantity, distribution, population and its components of endophytic fungi in different parts of Taxus yunnanensis existed differences. Among these endophytes, 4 species can produce taxol by the initial detection.

关 键 词：云南红豆杉 紫杉醇 内生真菌 分类 

分 类 号：S791.49[农业科学—林木遗传育种]