E.tenella吉林株单卵囊的分离及PCR鉴定  

Isolation of E.tenella JL Strain Single-oocyst and PCR Identification

作　　者：杨桂连[1,2] 李建华[1] 张西臣[1] 赵权[1,2] 宫鹏涛[1] 蔡亚南[1] 张国才[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《安徽农业科学》2009年第19期8893-8894,8900,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30671580;30170696)

摘　　要：[目的]为了获得E.tenella纯株,进行相关的分子生物学研究。[方法]对已经建立的球虫单卵囊分离技术进行改进,用分离的单卵囊胶囊接种雏鸡。同时应用PCR方法对收集的单卵囊分离株进行球虫种类鉴定。[结果]20只试验鸡中有15只粪便中分离到卵囊,感染成功率为75%。PCR扩增结果表明该单卵囊分离株为E.tenella。[结论]采用单卵囊胶囊进行接种,操作简便,既节省了接种时间,又降低了接种难度,且大大提高了接种成功率,为球虫的分子生物学研究提供了材料。[ Objective ] In order to obtain a purified strain of E. tenella, relative molecular biology researches were carried out. [ Method ] The single-oocyst isolation method was improved and the isolated single-oocyst which was put into capsule was fed to chickens. At the same time, the collected oocysts were identified by PCR method. [ Result] The oocysts were isolated from feces of 15 chickens among that of 20 chickens and the infection rate was 75%. The PCR results demonstrated that the single-oocyst strain was E. tenella. [ Conclusion] The inoculation of single-ooeyst capsule was simple, besides, this method did not only save time but also declined inoculation difficulty ,increased infection rate and provided good materials for biological research of coccidia.

关 键 词：E.TENELLA 单卵囊 PCR鉴定 

分 类 号：S852.72[农业科学—基础兽医学]