利用SSR分子标记分析苎麻居群的取样策略  

Sampling Strategy of Boehmeria nivea Population by SSR Molecular Markers

作　　者：赵中伟[1,2] 徐玲玲[1] 李同建[1] 廖亮[1,2] 潘其辉 赖占均 石庆华[2] 

机构地区：[1]九江学院生命科学学院,江西九江332000 [2]江西农业大学,江西南昌330045 [3]江西省麻类科学研究所,江西宜春336000

出　　处：《安徽农业科学》2009年第19期8901-8903,共3页Journal of Anhui Agricultural Sciences

基　　金：江西省自然科学基金项目(2008G2N0044);江西省教育厅科技项目(GJJ08431)

摘　　要：[目的]研究我国重要纤维植物苎麻的遗传多样性,确定苎麻居群的合理取样策略。[方法]以不同生境的5个居群为研究对象,每个居群分单株采集24株,分为4、8、12、16、20、24株6个取样梯度,利用8对SSR引物进行遗传多样性分析。[结果]①分布于不同生境的苎麻居群的等位基因数和遗传多样性指数各不同;②随着分析单位个体数目从4株增加到20株,各居群的多态位点数和遗传多样性指数均表现增大的趋势,但当分析单位的个体数目在20株以上时,多态位点数和遗传多样性指数基本不再发生变化;③当分析单位个体数目为20株时,各居群的多态位点数和遗传多样性指数分别包含了各自居群95%以上的遗传变异。[结论]在利用SSR技术进行苎麻居群遗传多样性研究中,以单个居群随机采集20株苎麻为最少分析单位个体数目。[ Objective ] The research aimed to investigate the genetic diversity of Chinese important fiber plant Boehmeria nivea effectively and determine a reasonable sampling strategy of ramie populations. [ Method ] Five populations distributed different habitats, which were evaluated by 8 pairs of simple sequence repeat （SSR） markers in 6 sample levels with different sample size. [ Result] ①The number of alleles and genetic diversity index were different from the populations distributed different habitats; ② The number of alleles and genetic diversity index increased along with the sampling size from 4 to 24 plants, and were not changed when the sample size over 20; ③ The number of alleles and genetic diversity index of 5 populations owned more than 95 percentage genetic variations of the total population when the sample size was 20. [ Conclusion] 20 individuals could represent the whole population of Boehmeria nivea based on the genetic diversity of SSR.

关 键 词：苎麻 SSR 遗传多样性 居群取样 

分 类 号：S563.1[农业科学—作物学]