冬枣花药愈伤组织的诱导与增殖  

Anther Callus Induction and Propagation of Ziziphus jujuba 'Dongzao'

作　　者：王娜[1,2] 刘孟军[1] 秦子禹[3] 刘晓光[1] 

机构地区：[1]河北农业大学中国枣研究中心,河北保定071001 [2]河北科技师范学院园艺园林系,河北秦皇岛066000 [3]河北科技师范学院生命科学系,河北秦皇岛066000

出　　处：《安徽农业科学》2009年第19期9006-9008,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省自然科学基金项目(C2007000452)

摘　　要：[目的]获得冬枣花药胚性愈伤组织。[方法]以冬枣花药为试材,研究了碳源种类、基本培养基、植物生长调节剂对冬枣花药愈伤组织诱导及增殖的影响。[结果]麦芽糖为诱导冬枣花药愈伤组织的最适碳源,MS为最适基本培养基,1.0 mg/L TDZ为最适诱导与增殖生长调节剂浓度。[结论]在MS+麦芽糖20 g/L+TDZ 1.0 mg/L的培养基中,冬枣花药愈伤组织诱导率最高,达86.8%。获得的愈伤组织可进行增殖,愈伤组织呈黄绿色颗粒状,为胚性愈伤。[Objective] The study aimed to obtain callus from Dongzao （Ziziphusjujuba Mill. ）. [Method] With anthers of Z. jujuba as test materials, the effects of carbon source kinds, basic medium, and plant growth regulator on the callus induction and propagation of Z. jujuba anthers were studied. [ Result ] The results indicated that maltose was the optimum carbon source, MS was the optimum basic medium, and 1.0 mg/L TDZ was the optimum concentration of growth regulator for callus induction and propagation. In the medium of MS ＋ 20 g/L maltose ＋ 1.0 mg/L TDZ, the callus induction rate was highest（86.8% ）. The induced callus was proliferated on MS medium supplemented with maltose 20 g/L ＋ TDZ 1.0 mg/L. And the callus was embryogenic callus with kelly and grain-shape.

关 键 词：冬枣 花药 愈伤组织诱导 愈伤组织增殖 

分 类 号：S665.1[农业科学—果树学]